GeneTex
United States (US)

Thymidylate synthase antibody

GTX103235_40744_WB_R_19011506_585.jpg
GTX103235_40744_IHC_2_19011506_842.jpg
GTX103235_40744_IHC_19011506_711.jpg
GTX103235_40744_IP_19011506_722.jpg
GTX103235_40744_WB_19011506_886.jpg
GTX103235_40744_20141107_WB_19011506_649.jpg
GTX103235_40744_IFA_19011506_510.jpg
GTX103235_40744_WB_M_19011506_489.jpg

Cat No. GTX103235

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Jurkat , Raji , THP-1 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat2

Predict Reactivity

Zebrafish, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Thymidylate synthetase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HST422 antibody, MGC88736 antibody, TMS antibody, TS antibody, TYMS antibody, TSase antibody, thymidylate synthase antibody, thymidylate synthetase antibody

Background

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103235_40744_WB_R_19011506_585.jpg

GTX103235 WB Image

Thymidylate synthetase antibody detects TYMS protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
10 % SDS-PAGE
Thymidylate synthetase antibody (GTX103235) dilution: 1:1000

GTX103235_40744_IHC_2_19011506_842.jpg

GTX103235 IHC-P Image

Thymidylate synthetase antibody detects Thymidylate synthetase protein at cytosol and nucleus on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
Thymidylate synthetase antibody (GTX103235) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103235_40744_IHC_19011506_711.jpg

GTX103235 IHC-P Image

Thymidylate synthetase antibody detects Thymidylate synthetase protein at cytosol and nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
Thymidylate synthetase antibody (GTX103235) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103235_40744_IP_19011506_722.jpg

GTX103235 IP Image

Thymidylate synthetase antibody immunoprecipitates TYMS protein in IP experiments. IP Sample: Jurkat whole cell lysate/extract A : 30 μg whole cell lysate/extract of TYMS protein expressing Jurkat cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of TYMS by 3 μg of thymidylate synthetase antibody (GTX103235) 10% SDS-PAGE The immunoprecipitated TYMS protein was detected by thymidylate synthetase antibody (GTX103235) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

GTX103235_40744_WB_19011506_886.jpg

GTX103235 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
C: THP-1
10% SDS PAGE
GTX103235 diluted at 1:1000

GTX103235_40744_20141107_WB_19011506_649.jpg

GTX103235 WB Image

Thymidylate synthetase antibody detects Thymidylate synthetase protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthetase antibody (GTX103235) diluted by 1:1000.

GTX103235_40744_IFA_19011506_510.jpg

GTX103235 ICC/IF Image

Thymidylate synthetase antibody detects Thymidylate synthetase protein at cytoplasm by immunofluorescent analysis.
Sample: A375 cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min.
Green: Thymidylate synthetase protein stained by Thymidylate synthetase antibody (GTX103235) diluted at 1:1000.
Blue: Hoechst 33342 staining.

GTX103235_40744_WB_M_19011506_489.jpg

GTX103235 WB Image

Thymidylate synthetase antibody detects TYMS protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg BCL-1 whole cell lysate/extract
F. 30 µg Raw264.7 whole cell lysate/extract
G. 30 µg C2C12 whole cell lysate/extract
10 % SDS-PAGE
Thymidylate synthetase antibody (GTX103235) dilution: 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169