Thymidylate synthetase antibody detects Thymidylate synthetase protein at cytosol and nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
Thymidylate synthetase antibody (GTX103235) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Thymidylate synthetase antibody immunoprecipitates TYMS protein in IP experiments. IP Sample: Jurkat whole cell lysate/extract A : 30 μg whole cell lysate/extract of TYMS protein expressing Jurkat cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of TYMS by 3 μg of thymidylate synthetase antibody (GTX103235) 10% SDS-PAGE The immunoprecipitated TYMS protein was detected by thymidylate synthetase antibody (GTX103235) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Thymidylate synthase antibody detects Thymidylate synthase protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Thymidylate synthase stained by Thymidylate synthase antibody (GTX103235) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Thymidylate synthetase antibody detects Thymidylate synthetase protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthetase antibody (GTX103235) diluted by 1:1000.

Thymidylate synthetase antibody detects TYMS protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw264.7 whole cell lysate/extract
G. 30 μg C2C12 whole cell lysate/extract
10 % SDS-PAGE
Thymidylate synthetase antibody (GTX103235) dilution: 1:1000

Thymidylate synthetase antibody detects Thymidylate synthetase protein at cytosol and nucleus on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
Thymidylate synthetase antibody (GTX103235) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
C: THP-1
10% SDS PAGE
GTX103235 diluted at 1:1000

Thymidylate synthetase antibody detects TYMS protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
10 % SDS-PAGE
Thymidylate synthetase antibody (GTX103235) dilution: 1:1000