APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
Jurkat , Raji , THP-1 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat2 , 293T , A431 , HeLa , HepG2
Predict Reactivity
Zebrafish, Dog, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Thymidylate synthase. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11179576
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
thymidylate synthetase , HST422 , TMS , TS
Background
Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX103235 WB Image
Thymidylate synthetase antibody detects TYMS protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
10 % SDS-PAGE
Thymidylate synthetase antibody (GTX103235) dilution: 1:1000
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GTX103235 WB Image
Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
C: THP-1
10% SDS PAGE
GTX103235 diluted at 1:1000
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GTX103235 IHC-P Image
Thymidylate synthetase antibody detects Thymidylate synthetase protein at cytosol and nucleus on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
Thymidylate synthetase antibody (GTX103235) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX103235 WB Image
Thymidylate synthetase antibody detects TYMS protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw264.7 whole cell lysate/extract
G. 30 μg C2C12 whole cell lysate/extract
10 % SDS-PAGE
Thymidylate synthetase antibody (GTX103235) dilution: 1:1000
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GTX103235 WB Image
Thymidylate synthetase antibody detects Thymidylate synthetase protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthetase antibody (GTX103235) diluted by 1:1000.
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GTX103235 ICC/IF Image
Thymidylate synthase antibody detects Thymidylate synthase protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Thymidylate synthase stained by Thymidylate synthase antibody (GTX103235) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX103235 IP Image
Thymidylate synthetase antibody immunoprecipitates TYMS protein in IP experiments. IP Sample: Jurkat whole cell lysate/extract A : 30 μg whole cell lysate/extract of TYMS protein expressing Jurkat cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of TYMS by 3 μg of thymidylate synthetase antibody (GTX103235) 10% SDS-PAGE The immunoprecipitated TYMS protein was detected by thymidylate synthetase antibody (GTX103235) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
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GTX103235 IHC-P Image
Thymidylate synthetase antibody detects Thymidylate synthetase protein at cytosol and nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
Thymidylate synthetase antibody (GTX103235) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
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