APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:2000 |
| 1:10000-1:50000 |
Not tested in other applications.
Calculated MW
Product Note
Detects Thymidylate Synthase by immunochemistry in proliferating cell cultures and tissues but does not stain non-proliferating cells. Normal colon mucosa shows weak staining. However, some colorectal cancer specimens show very strong staining.
PROPERTIES
Form
Liquid
Buffer
20mM Potassium Phosphate, 150mM NaCl
Preservative
0.01% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
10 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human Thymidylate synthase (36 kDa) produced in E.coli .
Purification
IgG fraction
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
thymidylate synthetase , HST422 , TMS , TS
Cellular Localization
Nucleus,Cytoplasm,Mitochondrion,Mitochondrion matrix,Mitochondrion inner membrane
Background
Because inhibition of Thymidylate Synthase (TS) prevents DNA synthesis and cell proliferation, the enzyme is an important target for cancer chemotherapeutic drugs, specifically the fluoropyrimidine group of antineoplastic drugs used to treat solid tumors.
Database
Research Area
DATA IMAGES
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GTX27398 Image
Anti-TS is shown to detect thymidylate synthase present in a HeLa cell extract. Each lane is estimated to contain 4 μg of protein. Lanes 1, 2 and 3 represent 1:2,000, 1:5,000 and 1:10,000 fold dilutions of the antibody. Detection was made using HRP Rabbit-anti-Sheep IgG diluted 1:1,000 and color development using TMB (TMBM-100) substrate.
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GTX27398 WB Image
WB analysis of HeLa whole cell lysate using GTX27398 Thymidylate synthase antibody.
Dilution :
Lane 1 : 1:2000
Lane 2 : 1:5000
Lane 3 : 1:10000
Loading : 4 μg
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GTX27398 WB Image
WB analysis of various samples using GTX27398 Thymidylate synthase antibody.
Lane 1 : Protein ladder
Lane 2 : HeLa whole cell lysate
Lane 3 : Molt-4 whole cell lysate
Loading : 10 μg
Dilution : 1 μg/mL
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REFERENCE
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REVIEW
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