WB analysis of various samples using GTX27398 Thymidylate synthase antibody.
Lane 1 : Protein ladder
Lane 2 : HeLa whole cell lysate
Lane 3 : Molt-4 whole cell lysate
Loading : 10 μg
Dilution : 1 μg/mL

WB analysis of HeLa whole cell lysate using GTX27398 Thymidylate synthase antibody.
Dilution :
Lane 1 : 1:2000
Lane 2 : 1:5000
Lane 3 : 1:10000
Loading : 4 μg

Anti-TS is shown to detect thymidylate synthase present in a HeLa cell extract. Each lane is estimated to contain 4 μg of protein. Lanes 1, 2 and 3 represent 1:2,000, 1:5,000 and 1:10,000 fold dilutions of the antibody. Detection was made using HRP Rabbit-anti-Sheep IgG diluted 1:1,000 and color development using TMB (TMBM-100) substrate.