APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1-2μg/ml |
| 1-2μg/ml|30 min at RT |
| 1-2μg/10⁶ cells |
| Assay dependent |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Human thyroid follicular cells
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
AITD3 , TG , TGN , thyroglobulin , Thyroglobulin
Cellular Localization
Secreted
Background
Thyroglobulin (Tg) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen. Mutations in this gene cause thyroid dyshormonogenesis, manifested as goiter, and are associated with moderate to severe congenital hypothyroidism. Polymorphisms in this gene are associated with susceptibility to autoimmune thyroid diseases (AITD) such as Graves disease and Hashimoto thryoiditis. [provided by RefSeq, Nov 2009]
Database
Research Area
DATA IMAGES
![Anti-Thyroglobulin antibody [SPM221] used in IHC (Paraffin sections) (IHC-P). GTX35116](/templates/default/js/lightbox/images/loading.gif "Anti-Thyroglobulin antibody [SPM221] used in IHC (Paraffin sections) (IHC-P). GTX35116")
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GTX35116 IHC-P Image
IHC-P analysis of human thyroid carcinoma tissue using GTX35116 Thyroglobulin antibody [SPM221].
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REFERENCE
There are currently no references for Thyroglobulin antibody [SPM221] (GTX35116). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Thyroglobulin antibody [SPM221] (GTX35116). Be the first to share your experience with this product.
![Anti-Thyroglobulin antibody [SPM221] used in IHC (Paraffin sections) (IHC-P). GTX35116](/upload/website/prouct_img/small/GTX35116/GTX35116_20200115_IHC-P_1241_20011518_985.jpg "Anti-Thyroglobulin antibody [SPM221] used in IHC (Paraffin sections) (IHC-P). GTX35116")
![Anti-Thyroglobulin antibody [SPM221] used in IHC (Paraffin sections) (IHC-P). GTX35116](/upload/website/prouct_img/normal/GTX35116/GTX35116_20200115_IHC-P_1241_20011518_985.jpg "Anti-Thyroglobulin antibody [SPM221] used in IHC (Paraffin sections) (IHC-P). GTX35116")