GeneTex

  • Country / Location Selection

United States (US)

Thyroglobulin antibody [SPM221]

Anti-Thyroglobulin antibody [SPM221] used in IHC (Paraffin sections) (IHC-P). GTX35116

Cat. No. GTX35116

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 SPM221

Isotype

 IgG1

Application

 WB, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($529)
See all Thyroglobulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
IHC-P 1-2μg/ml|30 min at RT
FACS 1-2μg/10⁶ cells
ELISA Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).
Not tested in other applications.

Calculated MW

305 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human thyroid follicular cells

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AITD3 , TG , TGN , thyroglobulin , Thyroglobulin

Cellular Localization

Secreted

Background

Thyroglobulin (Tg) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen. Mutations in this gene cause thyroid dyshormonogenesis, manifested as goiter, and are associated with moderate to severe congenital hypothyroidism. Polymorphisms in this gene are associated with susceptibility to autoimmune thyroid diseases (AITD) such as Graves disease and Hashimoto thryoiditis. [provided by RefSeq, Nov 2009]

Database

Research Area

DATA IMAGES

Anti-Thyroglobulin antibody [SPM221] used in IHC (Paraffin sections) (IHC-P). GTX35116

GTX35116 IHC-P Image

IHC-P analysis of human thyroid carcinoma tissue using GTX35116 Thyroglobulin antibody [SPM221].

REFERENCE

There are currently no references for Thyroglobulin antibody [SPM221] (GTX35116). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Thyroglobulin antibody [SPM221] (GTX35116). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529