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Thyroid Hormone Receptor alpha-2 antibody

Anti-Thyroid Hormone Receptor alpha-2 antibody used in IHC (Paraffin sections) (IHC-P). GTX82858
Anti-Thyroid Hormone Receptor alpha-2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82858
Anti-Thyroid Hormone Receptor alpha-2 antibody used in IHC (Paraffin sections) (IHC-P). GTX82858
Anti-Thyroid Hormone Receptor alpha-2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82858
Anti-Thyroid Hormone Receptor alpha-2 antibody used in IHC (Paraffin sections) (IHC-P). GTX82858
Anti-Thyroid Hormone Receptor alpha-2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82858
Anti-Thyroid Hormone Receptor alpha-2 antibody used in IHC (Paraffin sections) (IHC-P). GTX82858
Anti-Thyroid Hormone Receptor alpha-2 antibody used in IHC (Paraffin sections) (IHC-P). GTX82858
Anti-Thyroid Hormone Receptor alpha-2 antibody used in Western Blot (WB). GTX82858

Cat No. GTX82858

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
200 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:100
ICC/IF 1:10-1:200
IHC-P 1:100-1:500
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic Peptide: S(455) R A V C G E D D S S E A S S(469)

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

thyroid hormone receptor alpha , 6430529J03Rik , AW259572 , Erba , Nr1a1 , Rvr , T3R[a] , T3Ralpha , Thra1 , Thra2 , c-erbA-1 , c-erbA-alpha , c-erbAalpha

Cellular Localization

Nucleus

Background

The protein encoded by this gene is one of several nuclear hormone receptors that bind thyroid hormones such as triiodothyronine and thyroxine with high affinity. The encoded protein is a transcription factor that can activate or repress transcription. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-Thyroid Hormone Receptor alpha-2 antibody used in IHC (Paraffin sections) (IHC-P). GTX82858

GTX82858 IHC-P Image

IHC-P analysis of mouse thyroid tissue using GTX82858 Thyroid Hormone Receptor alpha-2 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-Thyroid Hormone Receptor alpha-2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82858

GTX82858 ICC/IF Image

ICC/IF analysis of A431 cells using GTX82858 Thyroid Hormone Receptor alpha-2 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC

Anti-Thyroid Hormone Receptor alpha-2 antibody used in IHC (Paraffin sections) (IHC-P). GTX82858

GTX82858 IHC-P Image

IHC-P analysis of human thyroid tissue using GTX82858 Thyroid Hormone Receptor alpha-2 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-Thyroid Hormone Receptor alpha-2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82858

GTX82858 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX82858 Thyroid Hormone Receptor alpha-2 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC

Anti-Thyroid Hormone Receptor alpha-2 antibody used in IHC (Paraffin sections) (IHC-P). GTX82858

GTX82858 IHC-P Image

IHC-P analysis of mouse thyroid tissue using GTX82858 Thyroid Hormone Receptor alpha-2 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:200

Anti-Thyroid Hormone Receptor alpha-2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82858

GTX82858 ICC/IF Image

ICC/IF analysis of C2C12 cells using GTX82858 Thyroid Hormone Receptor alpha-2 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC

Anti-Thyroid Hormone Receptor alpha-2 antibody used in IHC (Paraffin sections) (IHC-P). GTX82858

GTX82858 IHC-P Image

IHC-P analysis of human skin tissue using GTX82858 Thyroid Hormone Receptor alpha-2 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

Anti-Thyroid Hormone Receptor alpha-2 antibody used in IHC (Paraffin sections) (IHC-P). GTX82858

GTX82858 IHC-P Image

IHC-P analysis of human thyroid tissue using GTX82858 Thyroid Hormone Receptor alpha-2 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:200

Anti-Thyroid Hormone Receptor alpha-2 antibody used in Western Blot (WB). GTX82858

GTX82858 WB Image

WB analysis of 25 ug of mouse thyroid (Lane 1), NIH-3T3 (Lane 2) and A431 (Lane 3) cell lysates using GTX82858 Thyroid Hormone Receptor alpha-2 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf