APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100 |
| 1:10-1:200 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 0.1% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Synthetic Peptide: S(455) R A V C G E D D S S E A S S(469)
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
thyroid hormone receptor alpha , 6430529J03Rik , AW259572 , Erba , Nr1a1 , Rvr , T3R[a] , T3Ralpha , Thra1 , Thra2 , c-erbA-1 , c-erbA-alpha , c-erbAalpha
Cellular Localization
Nucleus
Background
The protein encoded by this gene is one of several nuclear hormone receptors that bind thyroid hormones such as triiodothyronine and thyroxine with high affinity. The encoded protein is a transcription factor that can activate or repress transcription. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Sep 2015]
Database
Research Area
DATA IMAGES
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GTX82858 IHC-P Image
IHC-P analysis of human skin tissue using GTX82858 Thyroid Hormone Receptor alpha-2 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100
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GTX82858 ICC/IF Image
ICC/IF analysis of A431 cells using GTX82858 Thyroid Hormone Receptor alpha-2 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC
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GTX82858 ICC/IF Image
ICC/IF analysis of NIH-3T3 cells using GTX82858 Thyroid Hormone Receptor alpha-2 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC
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GTX82858 IHC-P Image
IHC-P analysis of human thyroid tissue using GTX82858 Thyroid Hormone Receptor alpha-2 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:200
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GTX82858 WB Image
WB analysis of 25 ug of mouse thyroid (Lane 1), NIH-3T3 (Lane 2) and A431 (Lane 3) cell lysates using GTX82858 Thyroid Hormone Receptor alpha-2 antibody.
Dilution : 1:100
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GTX82858 IHC-P Image
IHC-P analysis of mouse thyroid tissue using GTX82858 Thyroid Hormone Receptor alpha-2 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:200
|
|
|
GTX82858 IHC-P Image
IHC-P analysis of human thyroid tissue using GTX82858 Thyroid Hormone Receptor alpha-2 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20
|
|
|
GTX82858 IHC-P Image
IHC-P analysis of mouse thyroid tissue using GTX82858 Thyroid Hormone Receptor alpha-2 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20
|
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|
GTX82858 ICC/IF Image
ICC/IF analysis of C2C12 cells using GTX82858 Thyroid Hormone Receptor alpha-2 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC
|
REFERENCE
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REVIEW
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