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Thyroid Hormone Receptor antibody [C3]

Anti-Thyroid Hormone Receptor antibody [C3] used in IHC (Paraffin sections) (IHC-P). GTX22743
Anti-Thyroid Hormone Receptor antibody [C3] used in IHC (Paraffin sections) (IHC-P). GTX22743
Anti-Thyroid Hormone Receptor antibody [C3] used in IHC (Paraffin sections) (IHC-P). GTX22743
Anti-Thyroid Hormone Receptor antibody [C3] used in IHC (Paraffin sections) (IHC-P). GTX22743
Anti-Thyroid Hormone Receptor antibody [C3] used in Western Blot (WB). GTX22743
Anti-Thyroid Hormone Receptor antibody [C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22743
Anti-Thyroid Hormone Receptor antibody [C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22743

Cat. No. GTX22743

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 C3

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IP, Gel supershift assays

Reactivity

 Human, Mouse, Rat, Xenopus, Primate
Package
100 μl ($399)
See all Thyroid Hormone Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1,000
ICC/IF 1:20
IHC-P 1:20
IP Assay dependent
Gel supershift assays Assay dependent
Not tested in other applications.

Product Note

This antibody recognizes TR beta-1 and also cross-reacts with TR alpha-1. This antibody recognizes an epitope in the hormone binding domain of hTR beta-1, amino acid residues 235-414.

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified fragment of human TR beta-1 corresponding to residues 201-456.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_374338

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

thyroid hormone receptor alpha , thyroid hormone receptor beta , AR7 , CHNG6 , EAR7 , ERB-T-1 , ERBA , ERBA1 , NR1A1 , THRA1 , THRA2 , c-ERBA-1 , C-ERBA-2 , C-ERBA-BETA , ERBA2 , GRTH , NR1A2 , PRTH , THR1 , THRB1 , THRB2

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Thyroid Hormone Receptor antibody [C3] used in IHC (Paraffin sections) (IHC-P). GTX22743

GTX22743 IHC-P Image

IHC-P analysis of mouse thyroid tissue using GTX22743 Thyroid Hormone Receptor antibody [C3].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-Thyroid Hormone Receptor antibody [C3] used in IHC (Paraffin sections) (IHC-P). GTX22743

GTX22743 IHC-P Image

IHC-P analysis of human thyroid tissue using GTX22743 Thyroid Hormone Receptor antibody [C3].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-Thyroid Hormone Receptor antibody [C3] used in IHC (Paraffin sections) (IHC-P). GTX22743

GTX22743 IHC-P Image

IHC-P analysis of human colon tissue using GTX22743 Thyroid Hormone Receptor antibody [C3].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-Thyroid Hormone Receptor antibody [C3] used in IHC (Paraffin sections) (IHC-P). GTX22743

GTX22743 IHC-P Image

IHC-P analysis of human thyroid tissue using GTX22743 Thyroid Hormone Receptor antibody [C3].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Anti-Thyroid Hormone Receptor antibody [C3] used in Western Blot (WB). GTX22743

GTX22743 WB Image

WB analysis of samples using GTX22743 Thyroid Hormone Receptor antibody [C3].

Anti-Thyroid Hormone Receptor antibody [C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22743

GTX22743 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22743 Thyroid Hormone Receptor antibody [C3]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-Thyroid Hormone Receptor antibody [C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22743

GTX22743 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX22743 Thyroid Hormone Receptor antibody [C3]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

REFERENCE

Application Reference

Hasebe T et al. Gen Comp Endocrinol 2020; 292 : 113441 Stem cell development involves divergent thyroid hormone receptor subtype expression and epigenetic modifications in the Xenopus metamorphosing intestine.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399