GeneTex
United States (US)

Thyroid Hormone Receptor beta (cleaved Arg327) antibody

THRB-cleaved-Arg327-antibody-GTX86914-WB-1_18121411_735.jpg

Cat No. GTX86914

Host Rabbit
Clonality Polyclonal
Application WB, ELISA
Reactivity Human
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
ELISA: 1:20000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

19 kDa. ( Note )

Specificity/Sensitivity

Thyroid Hormone Receptor beta (AP2,Cleaved-Arg327) Antibody detects endogenous levels of fragment of activated THRB resulting from cleavage adjacent to Arg327.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human Thyroid Hormone Receptor beta (cleaved Arg327).

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Thyroid Hormone Receptor Beta,C-Erba-2,C-Erba-Beta,Erba2,Grth,Nr1A2,Prth,Thr1,Thrb1,Thrb2,Thrb

Background

The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Mutations in this gene are known to be a cause of generalized thyroid hormone resistance (GTHR), a syndrome characterized by goiter and high levels of circulating thyroid hormone (T3-T4), with normal or slightly elevated thyroid stimulating hormone (TSH). Several alternatively spliced transcript variants encoding the same protein have been observed for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
THRB-cleaved-Arg327-antibody-GTX86914-WB-1_18121411_735.jpg

GTX86914 WB Image

Western blot analysis of extracts from Jurkat cells, treated with etoposide 25uM 24H, using THRB (AP2,Cleaved-Arg327) Antibody. The lane on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339