Tick-borne encephalitis virus (TBEV) Capsid antibody [HL4289]

Indirect ELISA analysis was performed by coating the plate with non-transfected and transfected 293T whole cell extracts. Coated protein was probed with Tick-borne encephalitis virus (TBEV) Capsid antibody [HL4289] (GTX643529) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Tick-borne encephalitis virus (TBEV) Capsid antibody [HL4289] (GTX643529) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Tick-borne encephalitis virus (TBEV) Capsid antibody [HL4289] detects Tick-borne encephalitis virus (TBEV) Capsid protein by immunofluorescent analysis.
Sample: Mock and TBEV (MOI:1, 24 hr) infected A549 cells were fixed in 4 % formaldehyde and permeabilized using PBS containing 0.5 % Triton X-100 and 20 mM glycine.
Red: Tick-borne encephalitis virus (TBEV) Capsid stained by Tick-borne encephalitis virus (TBEV) Capsid antibody [HL4289] (GTX643529) diluted at 1:500.
Blue: nuclear staining.

Non-infected (–) and infected (+) A549 whole cell extracts were separated by gradient gel, and the membrane was blotted with Tick-borne encephalitis virus (TBEV) Capsid antibody [HL4289] (GTX643529) diluted at 1:1000.