GeneTex
United States (US)

Timeless antibody [N1N2], N-term

GTX104334_WB_19011506_106.jpg
GTX104334_39925_IHC_19011506_959.jpg
GTX104334_IFA_19011506_210.jpg

Cat No. GTX104334

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

139 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Raji

Predict Reactivity

Mouse, Rat, Sheep, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human TIMELESS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Timeless Circadian Regulator,Tim,Tim1,Htim,Timeless

Cellular Localization

Nucleus

Background

Required for normal progression of S-phase. Involved in the circadian rhythm autoregulatory loop. Negatively regulates CLOCK-NPAS2/BMAL1-induced transactivation of PER1 possibly via translocation of PER1 into the nucleus. Promotes TIPIN nuclear localiZation. Involved in cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light. May also play an important role in epithelial cell morphogenesis and formation of branching tubules.

Database

Research Area

DATA IMAGES
GTX104334_WB_19011506_106.jpg

GTX104334 WB Image

Sample(30 ug whole cell lysate)
A:HeLa S3(GTX14654)
7.5% SDS PAGE
GTX104334 diluted at 1:500

GTX104334_39925_IHC_19011506_959.jpg

GTX104334 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SNU16 xenograft, using TIMELESS(GTX104334) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104334_IFA_19011506_210.jpg

GTX104334 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TIMELESS(GTX104334) antibody at 1:500 dilution.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169