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Timeless antibody [N1N2], N-term

Anti-Timeless antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX104334
Anti-Timeless antibody [N1N2], N-term used in Western Blot (WB). GTX104334
Anti-Timeless antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104334

Cat. No. GTX104334

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Timeless products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

139 kDa. ( Note )

Positive Control

HeLaS3

Predict Reactivity

Mouse, Rat, Sheep, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human TIMELESS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952248

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

timeless circadian regulator , TIM , TIM1 , hTIM

Cellular Localization

Nucleus

Background

Required for normal progression of S-phase. Involved in the circadian rhythm autoregulatory loop. Negatively regulates CLOCK-NPAS2/BMAL1-induced transactivation of PER1 possibly via translocation of PER1 into the nucleus. Promotes TIPIN nuclear localiZation. Involved in cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light. May also play an important role in epithelial cell morphogenesis and formation of branching tubules.

Database

Research Area

DATA IMAGES

Anti-Timeless antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX104334

GTX104334 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SNU16 xenograft, using TIMELESS(GTX104334) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Timeless antibody [N1N2], N-term used in Western Blot (WB). GTX104334

GTX104334 WB Image

Sample(30 ug whole cell lysate)
A:HeLa S3(GTX14654)
7.5% SDS PAGE
GTX104334 diluted at 1:500

Anti-Timeless antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104334

GTX104334 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TIMELESS(GTX104334) antibody at 1:500 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169