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Timeless antibody

Anti-Timeless antibody used in Western Blot (WB). GTX129604
Anti-Timeless antibody used in Western Blot (WB). GTX129604
Anti-Timeless antibody used in Immunoprecipitation (IP). GTX129604
Anti-Timeless antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129604

Cat. No. GTX129604

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Timeless products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:2000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

139 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Neuro2A , C8D30 , NIH-3T3 , Raw 264.7 , C2Cl2

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human TIMELESS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886050

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

timeless circadian regulator , TIM , TIM1 , hTIM

Cellular Localization

Nucleus

Background

Required for normal progression of S-phase. Involved in the circadian rhythm autoregulatory loop. Negatively regulates CLOCK-NPAS2/BMAL1-induced transactivation of PER1 possibly via translocation of PER1 into the nucleus. Promotes TIPIN nuclear localiZation. Involved in cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light. May also play an important role in epithelial cell morphogenesis and formation of branching tubules.

Database

Research Area

DATA IMAGES

Anti-Timeless antibody used in Western Blot (WB). GTX129604

GTX129604 WB Image

TIMELESS antibody detects TIMELESS protein by western blot analysis.
A. 30 μg Neuro2A whole cell extract
B. 30 μg C8D30 whole cell extract
C. 30 μg NIH-3T3 whole cell extract
D. 30 μg Raw 264.7 whole cell extract
E. 30 μg C2Cl2 whole cell extract
5 % SDS-PAGE
TIMELESS antibody (GTX129604) dilution: 1:1000

Anti-Timeless antibody used in Western Blot (WB). GTX129604

GTX129604 WB Image

TIMELESS antibody detects TIMELESS protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
5 % SDS-PAGE
TIMELESS antibody (GTX129604) dilution: 1:1000

Anti-Timeless antibody used in Immunoprecipitation (IP). GTX129604

GTX129604 IP Image

Immunoprecipitation of TIMELESS protein from 293T whole cell extracts using 5 μg of TIMELESS antibody (GTX129604).
Western blot analysis was performed using TIMELESS antibody (GTX129604).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Timeless antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129604

GTX129604 ICC/IF Image

TIMELESS antibody detects TIMELESS protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TIMELESS protein stained by TIMELESS antibody (GTX129604) diluted at 1:2000.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Truong LN et al. Proc Natl Acad Sci U S A 2013; 110 (19) : 2125834 Microhomology-mediated End Joining and Homologous Recombination share the initial end resection step to repair DNA double-strand breaks in mammalian cells.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169