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Tmbim1 antibody

Anti-Tmbim1 antibody used in Western Blot (WB). GTX48815

Cat. No. GTX48815

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
50 μg ($399)
See all TMBIM1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
IHC-P 10 μg/mL
ELISA 1:10000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein corresponding to the amino terminus of human TMBIM1 protein.

Purification

Protein A purified
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transmembrane BAX inhibitor motif containing 1 , LFG3 , MST100 , MSTP100 , PP1201 , RECS1

Cellular Localization

Membrane,Lysosome membrane,Endosome membrane

Background

TMBIM1 (Transμembrane BAX inhibitor μotif-containing protein 1) is a member of the 7 TMS (7 transmembrane domains) family of receptors known to mediate the activation of various transcription factors. TMBIM1 is identified as a novel modulator of NF-kB activation. A drosophila homolog of this protein (dNMDA1 with 40% homology to hTMBIM1) is reported to be unregulated during aging and oxidative stress.

Database

DATA IMAGES

Anti-Tmbim1 antibody used in Western Blot (WB). GTX48815

GTX48815 WB Image

Western blot using GeneTex protein A purified anti-TMBIM1 antibody (GTX48815) shows detection of exogenous TMBIM1 in lysates from HeLa cells transfected with pcDNA3-hTMBIM1 (lane 1). No staining is observed in lysates from mock transformed HeLa cells (lane 2). To date this antibody has shown the ability to recognize overexpressed TMBIM1 but not endogenous protein. The membrane was probed with the primary antibody at a 1:1,000 dilution at 4º C, overnight.

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399