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Tollip antibody [N2C3]

Anti-Tollip antibody [N2C3] used in Western Blot (WB). GTX116566
Anti-Tollip antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX116566
Anti-Tollip antibody [N2C3] used in Western Blot (WB). GTX116566
Anti-Tollip antibody [N2C3] used in Western Blot (WB). GTX116566
Anti-Tollip antibody [N2C3] used in Western Blot (WB). GTX116566
Anti-Tollip antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116566
Anti-Tollip antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116566

Cat. No. GTX116566

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

NT2D1 , IMR32

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Tollip. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11175682

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

toll interacting protein , IL-1RAcPIP

Cellular Localization

Cytoplasm

Background

This gene encodes a ubiquitin-binding protein that interacts with several Toll-like receptor (TLR) signaling cascade components. The encoded protein regulates inflammatory signaling and is involved in interleukin-1 receptor trafficking and in the turnover of IL1R-associated kinase. [supplied by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Tollip antibody [N2C3] used in Western Blot (WB). GTX116566

GTX116566 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
B: IMR32
12% SDS PAGE
GTX116566 diluted at 1:1000

Anti-Tollip antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX116566

GTX116566 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Tollip(GTX116566) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Tollip antibody [N2C3] used in Western Blot (WB). GTX116566

GTX116566 WB Image

The data was published in the journal EMBO J in 2020.PMID: 32311122

Anti-Tollip antibody [N2C3] used in Western Blot (WB). GTX116566

GTX116566 WB Image

The data was published in the journal EMBO J in 2020.PMID: 32311122

Anti-Tollip antibody [N2C3] used in Western Blot (WB). GTX116566

GTX116566 WB Image

The data was published in the journal EMBO J in 2020.PMID: 32311122

Anti-Tollip antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116566

GTX116566 ICC/IF Image

The data was published in the journal EMBO J in 2020.PMID: 32311122

Anti-Tollip antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116566

GTX116566 ICC/IF Image

The data was published in the journal EMBO J in 2020.PMID: 32311122

REFERENCE

REVIEW

Tollip antibody [N2C3] Cat. No. GTX116566

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 21-May-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : CL1-5 cells
Amount used : 40μg
Blocking : 5% milk, 25°C, 3Hr
Primary Antibody : 1:1000, 4°C, 9Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169