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Topoisomerase I antibody [SC69-03]

Recombinant antibody
Anti-Topoisomerase I antibody [SC69-03] used in Western Blot (WB). GTX01045
Anti-Topoisomerase I antibody [SC69-03] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01045
Anti-Topoisomerase I antibody [SC69-03] used in Flow cytometry (FACS). GTX01045
Anti-Topoisomerase I antibody [SC69-03] used in IHC (Paraffin sections) (IHC-P). GTX01045
Anti-Topoisomerase I antibody [SC69-03] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01045
Anti-Topoisomerase I antibody [SC69-03] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01045

Cat No. GTX01045

Host

Rabbit

Clonality

Monoclonal

Clone Name

SC69-03

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Mouse
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:5000
ICC/IF 1:50-1:500
IHC-P 1:50-1:200
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

91 kDa. ( Note )

Observed MW

100 kDa. The observed M.W. is based on the following publication. PMID: 16546964

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DNA topoisomerase I , TOP1 , TOPI , topoisomerase (DNA) I , Topoisomerase I , Topoisomerase 1

Cellular Localization

Nucleus,Nucleolus,Nucleoplasm

Background

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Topoisomerase I antibody [SC69-03] used in Western Blot (WB). GTX01045

GTX01045 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Topoisomerase I antibody [SC69-03] (GTX01045) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 16546964

Anti-Topoisomerase I antibody [SC69-03] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01045

GTX01045 ICC/IF Image

ICC/IF analysis of PFA-fixed 293 cells using GTX01045 Topoisomerase I antibody [SC69-03].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-Topoisomerase I antibody [SC69-03] used in Flow cytometry (FACS). GTX01045

GTX01045 FACS Image

FACS analysis of HepG2 cells using GTX01045 Topoisomerase I antibody [SC69-03].
Red : primary antibody
Black : unlabelled control
Dilution : 1:50

Anti-Topoisomerase I antibody [SC69-03] used in IHC (Paraffin sections) (IHC-P). GTX01045

GTX01045 IHC-P Image

IHC-P analysis of mouse brain tissue section using GTX01045 Topoisomerase I antibody [SC69-03].

Anti-Topoisomerase I antibody [SC69-03] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01045

GTX01045 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX01045 Topoisomerase I antibody [SC69-03].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-Topoisomerase I antibody [SC69-03] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01045

GTX01045 ICC/IF Image

ICC/IF analysis of PFA-fixed SHG-44 cells using GTX01045 Topoisomerase I antibody [SC69-03].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

REFERENCE

There are currently no references for Topoisomerase I antibody [SC69-03] (GTX01045). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Topoisomerase I antibody [SC69-03] (GTX01045). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 369