GeneTex
United States (US)

Topoisomerase I antibody

GTX64445_20180530_IP_18121411_281.jpg
GTX64445_20180530_WB_18121411_649.jpg
GTX64445_20180530_IHC-P_18121411_757.jpg

Cat No. GTX64445

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IP
Reactivity Human, Mouse
See all Topoisomerase I products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
IP 1:20 - 1:50
Not tested in other applications.

Calculated MW

91 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TOP1 (NP_003277.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

TOPI Antibody , TOP1 Antibody

Background

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
GTX64445_20180530_IP_18121411_281.jpg

GTX64445 IP Image

IP analysis of Jurkat cell lysate using GTX64445 Topoisomerase I antibody.
IP reaction : 3μg antibody / 150μg cell lysate
Dilution : 1:500
GTX64445_20180530_WB_18121411_649.jpg

GTX64445 WB Image

WB analysis of various samples using GTX64445 Topoisomerase I antibody.
Dilution : 1:1000
Loading : 25μg per lane
GTX64445_20180530_IHC-P_18121411_757.jpg

GTX64445 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX64445 Topoisomerase I antibody.
Dilution : 1:100
Package List Price ($)
$ 319