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Topoisomerase I antibody

Anti-Topoisomerase I antibody used in Immunoprecipitation (IP). GTX64445
Anti-Topoisomerase I antibody used in Western Blot (WB). GTX64445
Anti-Topoisomerase I antibody used in IHC (Paraffin sections) (IHC-P). GTX64445

Cat. No. GTX64445

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IP

Reactivity

Human, Mouse
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
IP 1:20 - 1:50
Not tested in other applications.

Calculated MW

91 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TOP1 (NP_003277.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DNA topoisomerase I , TOPI

Cellular Localization

nucleoplasm,Nucleolus

Background

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Topoisomerase I antibody used in Immunoprecipitation (IP). GTX64445

GTX64445 IP Image

IP analysis of Jurkat cell lysate using GTX64445 Topoisomerase I antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

Anti-Topoisomerase I antibody used in Western Blot (WB). GTX64445

GTX64445 WB Image

WB analysis of various sample lysates using GTX64445 Topoisomerase I antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Topoisomerase I antibody used in IHC (Paraffin sections) (IHC-P). GTX64445

GTX64445 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX64445 Topoisomerase I antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399