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Topoisomerase II alpha antibody [3F6]

Anti-Topoisomerase II alpha antibody [3F6] used in IHC (Paraffin sections) (IHC-P). GTX23111

Cat. No. GTX23111

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3F6

Isotype

 IgG1

Application

 IHC-P

Reactivity

 Human
Package
500 μl ($619)
See all Topoisomerase II alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100

Note :

IHC-P
The recommended fixative is 10% neutral-buffered formalin for paraffin-embedded tissue sections. Heat Induced Epitope Retreival (HIER) was performed in Epitope Retreival Solution pH6.
Not tested in other applications.

Calculated MW

174 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Tissue culture supernatant

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Antigen Species

Human

Immunogen

Prokaryotic recombinant protein corresponding to the C-terminal region of the topoisomerase II alpha molecule.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_370103

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA topoisomerase II alpha , TOP2 , TP2A

Cellular Localization

Cytoplasm , nucleoplasm

Background

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-Topoisomerase II alpha antibody [3F6] used in IHC (Paraffin sections) (IHC-P). GTX23111

GTX23111 IHC-P Image

IHC-P analysis of human bladder tumor using GTX23111 Topoisomerase II alpha antibody [3F6]. Note intense nuclear staining of malignant cells and occasional mitotic figures.

REFERENCE

Application Reference

Argenta PA et al. Gynecol Oncol 2013; 131 (2) : 368-73 Predicting response to the anti-estrogen * in recurrent ovarian cancer.
Application : IHC-P
Reactivity : Human
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REVIEW

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Package List Price ($)
$ 619