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Topoisomerase II alpha antibody, C-term

Anti-Topoisomerase II alpha antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX77787
Anti-Topoisomerase II alpha antibody, C-term used in Western Blot (WB). GTX77787

Cat. No. GTX77787

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all Topoisomerase II alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
IHC-P 2-10 ug/ml
Not tested in other applications.

Calculated MW

174 kDa. ( Note )

Positive Control

HepG2

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a C-terminal region of Human Topoisomerase II alpha

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_424735

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA topoisomerase II alpha , TOP2 , TP2A

Cellular Localization

Cytoplasm,nucleoplasm

Background

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-Topoisomerase II alpha antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX77787

GTX77787 IHC-P Image

IHC-P analysis of human liver tissue using GTX77787 Topoisomerase II alpha antibody at 4.0-8.0μg/ml.

Anti-Topoisomerase II alpha antibody, C-term used in Western Blot (WB). GTX77787

GTX77787 WB Image

WB analysis of HepG2 cells using GTX77787 Topoisomerase II alpha antibody at 1.25μg/ml.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399