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Topoisomerase II alpha antibody [C3], C-term

Anti-Topoisomerase II alpha antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100689
Anti-Topoisomerase II alpha antibody [C3], C-term used in Western Blot (WB). GTX100689
Anti-Topoisomerase II alpha antibody [C3], C-term used in Western Blot (WB). GTX100689

Cat. No. GTX100689

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Topoisomerase II alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

174 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Topoisomerase II alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952336

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA topoisomerase II alpha , TOP2 , TP2A

Cellular Localization

Cytoplasm , Nucleus , nucleoplasm

Background

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Topoisomerase II alpha antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100689

GTX100689 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SAS Xenograft , using Topoisomerase II alpha (GTX100689) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Topoisomerase II alpha antibody [C3], C-term used in Western Blot (WB). GTX100689

GTX100689 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
5% SDS PAGE
GTX100689 diluted at 1:3000

Anti-Topoisomerase II alpha antibody [C3], C-term used in Western Blot (WB). GTX100689

GTX100689 WB Image

The data was published in the journal Biomolecules in 2019. PMID: 31505885

REFERENCE

Application Reference

Li CJ et al. J Hepatocell Carcinoma 2023; 10 : 123-138
Application : WB
Reactivity : Human
Chen JC et al. Biomolecules 2019; 9 (9) In Vitro and In Silico Mechanistic Insights into miR-21-5p-Mediated Topoisomerase Drug Resistance in Human Colorectal Cancer Cells.
Application : WB
Reactivity : Human
Liu TP et al. R Soc Open Sci 2018; 5 (11) : 181321 Systematic polypharmacology and drug repurposing via an integrated L1000-based Connectivity Map database mining.
Application : WB
Reactivity : Human
Jeon KH et al. Biochim Biophys Acta 2018; 1862 (5) : 1126-1133 hyperactivated m-calpain affects acquisition of * resistance in breast cancer cells.
Application : WB
Reactivity : Human
Szu-Ting Lin et al. Cellular and Molecular Life Sciences 2015; 72 (12) : 2395-409 pgrmc1 contributes to *-induced chemoresistance in mes-sa uterine sarcoma.
Application : WB
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169