GeneTex
United States (US)

Topoisomerase II alpha antibody [C3], C-term

GTX100689_IHC_19011506_552.jpg
GTX100689_WB_19011506_498.jpg

Cat No. GTX100689

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

174 kDa. ( Note )

Positive Control

293T , A431 , H1299

Predict Reactivity

Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Topoisomerase II alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

TOP2 antibody, TP2A antibody, TOP2A antibody, DNA topoisomerase 2-alpha antibody, DNA gyrase antibody, "DNA topoisomerase II, 170 kD antibody", DNA topoisomerase (ATP-hydrolyzing) antibody, "DNA topoisomerase II, alpha isozyme antibody", topoisomerase (DNA) II alpha 170kDa antibody

Cellular Localization

Cytoplasm , Nucleus , nucleoplasm

Background

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq]

Database

DATA IMAGES
GTX100689_IHC_19011506_552.jpg

GTX100689 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SAS Xenograft , using Topoisomerase II alpha (GTX100689) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100689_WB_19011506_498.jpg

GTX100689 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
5% SDS PAGE
GTX100689 diluted at 1:3000

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169