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Topoisomerase II alpha antibody [MSVA-802R] HistoMAX™

Recombinant antibody HistoMAX
Anti-Topoisomerase II alpha antibody [MSVA-802R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04422
Anti-Topoisomerase II alpha antibody [MSVA-802R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04422
Anti-Topoisomerase II alpha antibody [MSVA-802R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04422

Cat. No. GTX04422

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 MSVA-802R

Isotype

 IgG

Application

 IHC-P

Reactivity

 Human
Package
500 μl ($739),
100 μl ($299)
See all Topoisomerase II alpha products
PRODUCT

Summary

This antibody was validated on 76 different Normal Tissues by IHC-P.

Go to Normal Tissue GalleryGo to Cancer Tissue Gallery

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:200

Note :

IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
Not tested in other applications.

Calculated MW

174 kDa. ( Note )

Positive Control

Colon: A fraction of cells, especially in the crypt basis should show a moderate to strong nuclear TOPO2A immunostaining.

Negative Control

Colon: Epithelial cells must not show any TOPO2A staining in the cytoplasm and in most nuclei of the surface epithelium.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA (Please contact us for PBS only format)

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Topoisomerase II alpha fragment (aa1352-1493)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA topoisomerase II alpha , TOP2 , TP2A

Cellular Localization

Cytoplasm , nucleoplasm

Background

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-Topoisomerase II alpha antibody [MSVA-802R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04422

GTX04422 IHC-P Image

IHC-P analysis of human testis tissue using GTX04422 Topoisomerase II alpha antibody [MSVA-802R] HistoMAX™.
Spermatocyte of the testis subject to meiosis is the only normal cell type with a consistent strong topoisomerase II alpha expression in virtually all cells.

Anti-Topoisomerase II alpha antibody [MSVA-802R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04422

GTX04422 IHC-P Image

IHC-P analysis of human colorectal adenocarcinoma (COAD) tissue using GTX04422 Topoisomerase II alpha antibody [MSVA-802R] HistoMAX™.
Colorectal adenocarcinoma with TOPO2A immunostaining in 50% of tumor cells.

Anti-Topoisomerase II alpha antibody [MSVA-802R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04422

GTX04422 IHC-P Image

IHC-P analysis of human mucosa from appendix tissue using GTX04422 Topoisomerase II alpha antibody [MSVA-802R] HistoMAX™.
Topoisomerase II alpha positive cells in the appendix mucosa reflect proliferating cells.

REFERENCE

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REVIEW

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Package List Price ($)
$ 739
$ 299