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Topoisomerase II alpha antibody [TOP2A/1361]

KO/KD Orthogonal
Anti-Topoisomerase II alpha antibody [TOP2A/1361] used in Western Blot (WB). GTX35137
Anti-Topoisomerase II alpha antibody [TOP2A/1361] used in IHC (Paraffin sections) (IHC-P). GTX35137
Anti-Topoisomerase II alpha antibody [TOP2A/1361] used in Western Blot (WB). GTX35137
Anti-Topoisomerase II alpha antibody [TOP2A/1361] used in Protein Array (Protein Array). GTX35137
Anti-Topoisomerase II alpha antibody [TOP2A/1361] used in IHC (Paraffin sections) (IHC-P). GTX35137
Anti-Topoisomerase II alpha antibody [TOP2A/1361] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35137
Anti-Topoisomerase II alpha antibody [TOP2A/1361] used in Western Blot (WB). GTX35137
Anti-Topoisomerase II alpha antibody [TOP2A/1361] used in Western Blot (WB). GTX35137
Anti-Topoisomerase II alpha antibody [TOP2A/1361] used in Western Blot (WB). GTX35137

Cat. No. GTX35137

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 TOP2A/1361

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, Protein Array

Reactivity

 Human, Mouse
Package
100 μg ($529)
See all Topoisomerase II alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 min at RT
Protein Array Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

174 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Topoisomerase II alpha fragment (aa1352-1493)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TOP2 , TOP2A , TP2A , topoisomerase (DNA) II alpha , Topoisomerase II alpha , Topo II alpha , DNA topoisomerase II alpha

Cellular Localization

Cytoplasm,Nucleus,Nucleoplasm

Background

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-Topoisomerase II alpha antibody [TOP2A/1361] used in Western Blot (WB). GTX35137

GTX35137 WB Image

WB analysis of HepG2, HeLa, and 3T3 cell lysates using GTX35137 Topoisomerase II alpha antibody [TOP2A/1361].

Anti-Topoisomerase II alpha antibody [TOP2A/1361] used in IHC (Paraffin sections) (IHC-P). GTX35137

GTX35137 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX35137 Topoisomerase II alpha antibody [TOP2A/1361].

Anti-Topoisomerase II alpha antibody [TOP2A/1361] used in Western Blot (WB). GTX35137

GTX35137 WB Image

WB analysis of HeLa cell lysate using GTX35137 Topoisomerase II alpha antibody [TOP2A/1361].

Anti-Topoisomerase II alpha antibody [TOP2A/1361] used in Protein Array (Protein Array). GTX35137

GTX35137 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using Topoisomerase II alpha Mouse Monoclonal Antibody (TOP2A/1361). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

Anti-Topoisomerase II alpha antibody [TOP2A/1361] used in IHC (Paraffin sections) (IHC-P). GTX35137

GTX35137 IHC-P Image

IHC-P analysis of human bladder carcinoma tissue using GTX35137 Topoisomerase II alpha antibody [TOP2A/1361].

Anti-Topoisomerase II alpha antibody [TOP2A/1361] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35137

GTX35137 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX35137 Topoisomerase II alpha antibody [TOP2A/1361].
Green : Primary antibody
Red : nucleus

Anti-Topoisomerase II alpha antibody [TOP2A/1361] used in Western Blot (WB). GTX35137

GTX35137 WB Image

Orthogonal

The data was published in the 2021 in Elife. PMID: 34180392

Anti-Topoisomerase II alpha antibody [TOP2A/1361] used in Western Blot (WB). GTX35137

GTX35137 WB Image

KO/KD Orthogonal

The data was published in the 2021 in Elife. PMID: 34180392

Anti-Topoisomerase II alpha antibody [TOP2A/1361] used in Western Blot (WB). GTX35137

GTX35137 WB Image

Orthogonal

The data was published in the 2021 in Elife. PMID: 34180392

REFERENCE

Application Reference

LH Giang et al. J Exp Clin Cancer Res 2023; 42 (1) : 346 Targeting of RRM2 suppresses DNA damage response and activates apoptosis in atypical teratoid rhabdoid tumor
Application : WB
Reactivity : Human
Bossaert M et al. Elife 2021; 10 Transcription-associated topoisomerase 2α (TOP2A) activity is a major effector of cytotoxicity induced by G-quadruplex ligands.
Application : WB, ICC/IF
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529