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Topoisomerase II alpha antibody [TOP2A/4397R]

Anti-Topoisomerase II alpha antibody [TOP2A/4397R] used in IHC (Paraffin sections) (IHC-P). GTX02727
Anti-Topoisomerase II alpha antibody [TOP2A/4397R] used in IHC (Paraffin sections) (IHC-P). GTX02727

Cat. No. GTX02727

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 TOP2A/4397R

Isotype

 IgG

Application

 IHC-P

Reactivity

 Human
Package
100 μg ($529)
See all Topoisomerase II alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1-2 μg/ml

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

174 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

200 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to human Topo II α residues within aa1431-1531

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA topoisomerase II alpha , TOP2 , TP2A

Cellular Localization

Cytoplasm , nucleoplasm

Background

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-Topoisomerase II alpha antibody [TOP2A/4397R] used in IHC (Paraffin sections) (IHC-P). GTX02727

GTX02727 IHC-P Image

IHC-P analysis of human lymph node tissue section using GTX02727 Topoisomerase II alpha antibody [TOP2A/4397R].

Anti-Topoisomerase II alpha antibody [TOP2A/4397R] used in IHC (Paraffin sections) (IHC-P). GTX02727

GTX02727 IHC-P Image

IHC-P analysis of human tonsil tissue section using GTX02727 Topoisomerase II alpha antibody [TOP2A/4397R].

REFERENCE

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REVIEW

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Package List Price ($)
$ 529