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Topoisomerase II beta antibody [C3], C-term

Anti-Topoisomerase II beta antibody [C3], C-term used in Immunoprecipitation (IP). GTX102640
Anti-Topoisomerase II beta antibody [C3], C-term used in Western Blot (WB). GTX102640
Anti-Topoisomerase II beta antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX102640
Anti-Topoisomerase II beta antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102640
Anti-Topoisomerase II beta antibody [C3], C-term used in Western Blot (WB). GTX102640

Cat No. GTX102640

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

183 kDa. ( Note )

Positive Control

Jurkat , K562 , THP-1

Predict Reactivity

Rat, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Topoisomerase II beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DNA topoisomerase II beta , TOPIIB , top2beta

Cellular Localization

Cytoplasm , Nucleus , nucleolus

Background

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing of this gene results in two transcript variants; however, the second variant has not yet been fully described. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Topoisomerase II beta antibody [C3], C-term used in Immunoprecipitation (IP). GTX102640

GTX102640 IP Image

Topoisomerase II beta antibody immunoprecipitates Topoisomerase II beta protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Topoisomerase II beta protein by 2 μg of Topoisomerase II beta antibody (GTX102640) 5% SDS-PAGE The immunoprecipitated Topoisomerase II beta protein was detected by Topoisomerase II beta antibody (GTX102640) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Topoisomerase II beta antibody [C3], C-term used in Western Blot (WB). GTX102640

GTX102640 WB Image

Sample (30 µg of whole cell lysate)
A: Jurkat
5% SDS PAGE
GTX102640 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Topoisomerase II beta antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX102640

GTX102640 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using Topoisomerase II beta(GTX102640) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Topoisomerase II beta antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102640

GTX102640 ICC/IF Image

Topoisomerase II beta antibody [C3], C-term detects Topoisomerase II beta protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Topoisomerase II beta stained by Topoisomerase II beta antibody [C3], C-term (GTX102640) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10 μm.

Anti-Topoisomerase II beta antibody [C3], C-term used in Western Blot (WB). GTX102640

GTX102640 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Topoisomerase II beta antibody [C3], C-term (GTX102640) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169