GeneTex
United States (US)

Tp63 antibody

GTX124660_39918_20141222_IHC-Wm_Z_2_18121410_915.jpg
GTX124660_41143_20150410_WB_Z_muscle_18121410_130.jpg
GTX124660_39918_IHC-Wm_Z_18121410_732.jpg
GTX124660_43138_20180703_IHC-WM_Z_18121410_117.jpg
GTX124660_39918_IHC_Z_18121410_973.jpg

Cat No. GTX124660

Reference 2
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Fr, IHC-Wm, IHC
Reactivity Zebrafish
See all zebrafish p63 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P Assay dependent
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
IHC 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

zebrafish muscle
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.34 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Tp63

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tumor Protein P63,Dnp63,Fc89F04,Id:Ibd3516,P63,Tp73L,Wu:Fc89F04,Wu:Fk85H07,Wu:Fk88B02,Tp63

Research Area

DATA IMAGES
GTX124660_39918_20141222_IHC-Wm_Z_2_18121410_915.jpg

GTX124660 IHC-Wm Image

Tp63 antibody detects Tp63 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Tp63 antibody (GTX124660) dilution: 1:100.
GTX124660_41143_20150410_WB_Z_muscle_18121410_130.jpg

GTX124660 WB Image

p63 antibody detects p63 protein by western blot analysis. Zebrafish tissue extracts (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with p63 antibody (GTX124660) at a dilution of 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX124660_39918_IHC-Wm_Z_18121410_732.jpg

GTX124660 IHC-Wm Image

Tp63 antibody detects Tp63 protein at nucleus on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
Tp63 antibody (GTX124660) dilution: 1:200.
GTX124660_43138_20180703_IHC-WM_Z_18121410_117.jpg

GTX124660 IHC-Wm Image

Tp63 antibody detects Tp63 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Tp63 stained by Tp63 antibody (GTX124660) diluted at 1:100.
GTX124660_39918_IHC_Z_18121410_973.jpg

GTX124660 IHC Image

Immunohistochemical analysis of agarose-embedded zebrafish embryo, using tp63 (GTX124660) antibody at 1:500 dilution. (This image was provided courtesy of the Schilling Lab at UC, Irvine.)
REFERENCE

Application Reference

Kimelman D et al. Elife 2017; 6 Regulation of posterior body and epidermal morphogenesis in Zebrafish by localized Yap1 and Wwtr1.
Application : IHC-Fr
Reactivity : Zebrafish
Bloomquist, Ryan F et al. 0 2015; Developmental plasticity of stem cells in teeth and taste bud renewal.
Application : IHC-P
Reactivity : Zebrafish
Submit a Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 389
$ 199