Tp63 antibody

Cat No. GTX124660

Reference 5

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, IHC-Fr, IHC-Wm, ChIP assay, IHC
Reactivity	Zebrafish, Fish
Package	200 μl ($389), 50 μl ($199)

See all zebrafish p63 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
IHC-P	Assay dependent
IHC-Fr	Assay dependent
IHC-Wm	1:100-1:500
ChIP assay	Assay dependent
IHC	1:100-1:1000

Not tested in other applications.

Positive Control

zebrafish muscle

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.34 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Tp63

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tumor protein p63 , DNp63 , fc89f04 , id:ibd3516 , p63 , tp73l , wu:fc89f04 , wu:fk85h07 , wu:fk88b02

Database

Gene ID: 260407 tp63

Research Area

DATA IMAGES

GTX124660 IHC-Wm Image

Tp63 antibody detects Tp63 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Tp63 antibody (GTX124660) dilution: 1:100.

GTX124660 WB Image

p63 antibody detects p63 protein by western blot analysis. Zebrafish tissue extracts (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with p63 antibody (GTX124660) at a dilution of 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124660 IHC-Wm Image

Tp63 antibody detects Tp63 protein at nucleus on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
Tp63 antibody (GTX124660) dilution: 1:200.

GTX124660 IHC-Wm Image

Tp63 antibody detects Tp63 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Tp63 stained by Tp63 antibody (GTX124660) diluted at 1:100.

GTX124660 IHC Image

Immunohistochemical analysis of agarose-embedded zebrafish embryo, using tp63 (GTX124660) antibody at 1:500 dilution. (This image was provided courtesy of the Schilling Lab at UC, Irvine.)

REFERENCE