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Tp63 antibody

Anti-Tp63 antibody used in IHC (Whole mount) (IHC-Wm). GTX124660
Anti-Tp63 antibody used in Western Blot (WB). GTX124660
Anti-Tp63 antibody used in IHC (Whole mount) (IHC-Wm). GTX124660
Anti-Tp63 antibody used in IHC (Whole mount) (IHC-Wm). GTX124660
Anti-Tp63 antibody used in Immunohistochemistry (IHC). GTX124660

Cat. No. GTX124660

Reference 10
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr, IHC-Wm, ChIP assay, IHC

Reactivity

 Zebrafish, Fish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish p63 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
ChIP assay Assay dependent
IHC 1:100-1:1000
Not tested in other applications.

Positive Control

zebrafish muscle

Predict Reactivity

Human (100%)| Rat (99%)| Zebrafish (94%)| Bovine (100%)| Chicken (96%)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.31 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Tp63

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11175363

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tumor protein p63 , DNp63 , fc89f04 , id:ibd3516 , p63 , tp73l , wu:fc89f04 , wu:fk85h07 , wu:fk88b02

Database

Research Area

DATA IMAGES

Anti-Tp63 antibody used in IHC (Whole mount) (IHC-Wm). GTX124660

GTX124660 IHC-Wm Image

Tp63 antibody detects Tp63 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Tp63 antibody (GTX124660) dilution: 1:100.
Anti-Tp63 antibody used in Western Blot (WB). GTX124660

GTX124660 WB Image

p63 antibody detects p63 protein by western blot analysis. Zebrafish tissue extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with p63 antibody (GTX124660) at a dilution of 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Tp63 antibody used in IHC (Whole mount) (IHC-Wm). GTX124660

GTX124660 IHC-Wm Image

Tp63 antibody detects Tp63 protein at nucleus on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
Tp63 antibody (GTX124660) dilution: 1:200.
Anti-Tp63 antibody used in IHC (Whole mount) (IHC-Wm). GTX124660

GTX124660 IHC-Wm Image

Tp63 antibody detects Tp63 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Tp63 stained by Tp63 antibody (GTX124660) diluted at 1:100.

Anti-Tp63 antibody used in Immunohistochemistry (IHC). GTX124660

GTX124660 IHC Image

Immunohistochemical analysis of agarose-embedded zebrafish embryo, using tp63 (GTX124660) antibody at 1:500 dilution. (This image was provided courtesy of the Schilling Lab at UC, Irvine.)

REFERENCE

Application Reference

Sebastian Wurster et al. Cell Rep 2021; 34 (12) : 108896 EGF-mediated suppression of cell extrusion during mucosal damage attenuates opportunistic fungal invasion.
Application : IHC
Reactivity : Zebrafish
Fadul J et al. Nat Commun 2021; 12 (1) : 7180 KRas-transformed epithelia cells invade and partially dedifferentiate by basal cell extrusion.
Application : IHC-Wm
Reactivity : Zebrafish
Brock CK et al. Nat Commun 2019; 10 (1) : 1044 Stem cell proliferation is induced by apoptotic bodies from dying cells during epithelial tissue maintenance.
K?nig D et al. Front Mol Neurosci 2019; 12 : 227 Distribution and Restoration of Serotonin-Immunoreactive Paraneuronal Cells During Caudal Fin Regeneration in Zebrafish.
Application : IHC-Wm
Bloomquist RF et al. Proc Natl Acad Sci U S A 2019; 116 (36) : 17858-17866 Developmental plasticity of epithelial stem cells in tooth and taste bud renewal.
Application : IHC-P
Reactivity : Fish
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Submit a Reference

REVIEW

Tp63 antibody Cat. No. GTX124660

Rating
( Average 5 based on 2 users reviews)
Application
IHC (Whole mount)(IHC-Wm)
( Average 5 based on 2 users reviews)
Anti-Tp63 antibody used in IHC (Whole mount) (IHC-Wm). GTX124660
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : IHC-Wm
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the 2019 in Front Mol Neurosci. PMID: 31616250
Anti-Tp63 antibody used in IHC (Whole mount) (IHC-Wm). GTX124660
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Wm
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2019. PMID: 30837472
Submit a Review
Application Reference
Sebastian Wurster et al. Cell Rep 2021; 34 (12):108896 EGF-mediated suppression of cell extrusion during mucosal damage attenuates opportunistic fungal invasion.
Application : IHC
Reactivity : Zebrafish
Fadul J et al. Nat Commun 2021; 12 (1):7180 KRas-transformed epithelia cells invade and partially dedifferentiate by basal cell extrusion.
Application : IHC-Wm
Reactivity : Zebrafish
Brock CK et al. Nat Commun 2019; 10 (1):1044 Stem cell proliferation is induced by apoptotic bodies from dying cells during epithelial tissue maintenance.
K?nig D et al. Front Mol Neurosci 2019; 12:227 Distribution and Restoration of Serotonin-Immunoreactive Paraneuronal Cells During Caudal Fin Regeneration in Zebrafish.
Application : IHC-Wm
Bloomquist RF et al. Proc Natl Acad Sci U S A 2019; 116 (36):17858-17866 Developmental plasticity of epithelial stem cells in tooth and taste bud renewal.
Application : IHC-P
Reactivity : Fish
D?sir?e K?nig et al. bioRxiv 2019; (Epub) Distribution and restoration of serotonin-immunoreactive paraneuronal cells during caudal fin regeneration in zebrafish.
Application : IHC-Wm
Reactivity : Zebrafish
Santos-Pereira JM et al. Nat Commun 2019; 10 (1):3049 Pioneer and repressive functions of p63 during zebrafish embryonic ectoderm specification.
Application : IHC-Wm, ChIP assay
Reactivity : Zebrafish
Schepis A et al. J Cell Biol 2018; 217 (3):1097-1112 Protease signaling regulates apical cell extrusion, cell contacts, and proliferation in epithelia.
Kimelman D et al. Elife 2017; 6 Regulation of posterior body and epidermal morphogenesis in Zebrafish by localized Yap1 and Wwtr1.
Application : IHC-Fr
Reactivity : Zebrafish
Bloomquist, Ryan F et al. 0 2015; Developmental plasticity of stem cells in teeth and taste bud renewal.
Application : IHC-P
Reactivity : Zebrafish
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169