GeneTex
United States (US)

Transaldolase antibody [N2C3]

GTX114956_40499_20180718_ICC_IF_18121410_431.jpg
GTX114956_40499_WB_18121410_837.jpg

Cat No. GTX114956

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

U87-MG , MCF-7

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Transaldolase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Transaldolase 1,Tal,Tal-H,Taldor,Talh,Taldo1

Cellular Localization

Cytoplasm

Background

Transaldolase 1 is a key enzyme of the nonoxidative pentose phosphate pathway providing ribose-5-phosphate for nucleic acid synthesis and NADPH for lipid biosynthesis. This pathway can also maintain glutathione at a reduced state and thus protect sulfhydryl groups and cellular integrity from oxygen radicals. The functional gene of transaldolase 1 is located on chromosome 11 and a pseudogene is identified on chromosome 1 but there are conflicting map locations. The second and third exon of this gene were developed by insertion of a retrotransposable element. This gene is thought to be involved in multiple sclerosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX114956_40499_20180718_ICC_IF_18121410_431.jpg

GTX114956 ICC/IF Image

Transaldolase antibody [N2C3] detects Transaldolase protein at cytoplasm by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Transaldolase stained by Transaldolase antibody [N2C3] (GTX114956) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX114956_40499_WB_18121410_837.jpg

GTX114956 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
B: MCF-7
10% SDS PAGE
GTX114956 diluted at 1:2000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169