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Transaldolase antibody

Orthogonal
Anti-Transaldolase antibody used in Western Blot (WB). GTX102076
Anti-Transaldolase antibody used in Western Blot (WB). GTX102076
Anti-Transaldolase antibody used in IHC (Paraffin sections) (IHC-P). GTX102076
Anti-Transaldolase antibody used in IHC (Paraffin sections) (IHC-P). GTX102076

Cat. No. GTX102076

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Transaldolase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

A431 , Mouse brain

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

6.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Transaldolase. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_1952136

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transaldolase 1 , TAL , TAL-H , TALDOR , TALH

Cellular Localization

Cytoplasm

Background

Transaldolase 1 is a key enzyme of the nonoxidative pentose phosphate pathway providing ribose-5-phosphate for nucleic acid synthesis and NADPH for lipid biosynthesis. This pathway can also maintain glutathione at a reduced state and thus protect sulfhydryl groups and cellular integrity from oxygen radicals. The functional gene of transaldolase 1 is located on chromosome 11 and a pseudogene is identified on chromosome 1 but there are conflicting map locations. The second and third exon of this gene were developed by insertion of a retrotransposable element. This gene is thought to be involved in multiple sclerosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Transaldolase antibody used in Western Blot (WB). GTX102076

GTX102076 WB Image

Sample (30 μg of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
GTX102076 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Transaldolase antibody used in Western Blot (WB). GTX102076

GTX102076 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX102076 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Transaldolase antibody used in IHC (Paraffin sections) (IHC-P). GTX102076

GTX102076 IHC-P Image

Transaldolase antibody detects TALDO1 protein at nucleus on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
Transaldolase antibody (GTX102076) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Transaldolase antibody used in IHC (Paraffin sections) (IHC-P). GTX102076

GTX102076 IHC-P Image

Orthogonal

The data was published in the journal Nat Commun in 2018.PMID: 30323337

REFERENCE

Application Reference

Chen JF et al. J Pharm Biomed Anal 2020; 187 : 113142 Proteomic analysis of Antrodia Cinnamomea-induced ER stress in liver cancer cells.
Application : WB
Reactivity : Human
Ding Y et al. Nat Commun 2018; 9 (1) : 4274 Synthetic lethality between HER2 and transaldolase in intrinsically resistant HER2-positive breast cancers.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169