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Transferrin antibody [HL2431]

Recombinant antibody Orthogonal
Anti-Transferrin antibody [HL2431] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638747
Anti-Transferrin antibody [HL2431] used in Western Blot (WB). GTX638747
Anti-Transferrin antibody [HL2431] used in IHC (Paraffin sections) (IHC-P). GTX638747
Anti-Transferrin antibody [HL2431] used in Western Blot (WB). GTX638747

Cat. No. GTX638747

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2431

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Transferrin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human Transferrin.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transferrin , HEL-S-71p , PRO1557 , PRO2086 , TFQTL1

Cellular Localization

Secreted

Background

This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. [provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES

Anti-Transferrin antibody [HL2431] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638747

GTX638747 ICC/IF Image

Transferrin antibody [HL2431] detects Transferrin protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in MeOH.
Green: Transferrin stained by Transferrin antibody [HL2431] (GTX638747) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Transferrin antibody [HL2431] used in Western Blot (WB). GTX638747

GTX638747 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Transferrin antibody [HL2431] (GTX638747) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Transferrin antibody [HL2431] used in IHC (Paraffin sections) (IHC-P). GTX638747

GTX638747 IHC-P Image

Transferrin antibody [HL2431] detects secreted Transferrin protein by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatocellular carcinoma.
Transferrin stained by Transferrin antibody [HL2431] (GTX638747) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Transferrin antibody [HL2431] used in Western Blot (WB). GTX638747

GTX638747 WB Image

Human plasma (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Transferrin antibody [HL2431] (GTX638747) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations