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Transferrin antibody [N3C2], Internal

Orthogonal
Anti-Transferrin antibody [N3C2], Internal used in Western Blot (WB). GTX112729
Anti-Transferrin antibody [N3C2], Internal used in Western Blot (WB). GTX112729
Anti-Transferrin antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX112729
Anti-Transferrin antibody [N3C2], Internal used in Western Blot (WB). GTX112729
Anti-Transferrin antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112729

Cat. No. GTX112729

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all Transferrin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Positive Control

rat plasma

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.51 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Transferrin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952199

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transferrin , HEL-S-71p , PRO1557 , PRO2086 , TFQTL1

Cellular Localization

Secreted

Background

The protein encoded by this gene is a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds 1 ion of ferric iron. The function of this encoded protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. In addition to its function in iron transport, this protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter/allergins from serum. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Transferrin antibody [N3C2], Internal used in Western Blot (WB). GTX112729

GTX112729 WB Image

Human plasma (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Transferrin antibody [N3C2], Internal (GTX112729) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Transferrin antibody [N3C2], Internal used in Western Blot (WB). GTX112729

GTX112729 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Transferrin antibody [N3C2], Internal (GTX112729) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Transferrin antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX112729

GTX112729 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using Transferrin(GTX112729) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Transferrin antibody [N3C2], Internal used in Western Blot (WB). GTX112729

GTX112729 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Transferrin antibody [N3C2], Internal (GTX112729) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Transferrin antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112729

GTX112729 ICC/IF Image

Immunofluorescence analysis of methanol-fixed Hep3B, using Transferrin(GTX112729) antibody at 1:500 dilution.

REFERENCE

Application Reference

Chih-Hsin Hsu et al. Int J Mol Sci 2021; 22 (19) miR-29a-3p/THBS2 Axis Regulates PAH-Induced Cardiac Fibrosis.
Application : WB
Reactivity : Human
Marziali LN et al. Glia 2016; 64 (11) : 1879-91 Combined effects of transferrin and thyroid hormone during oligodendrogenesis In vitro.
Application : WB
Reactivity : Rat
Lin CL et al. PLoS One 2013; 8 (5) : e65456 An occult hepatitis B-derived hepatoma cell line carrying persistent nuclear viral DNA and permissive for exogenous hepatitis B virus infection.
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169