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Transferrin antibody [N3C3]

Orthogonal
Anti-Transferrin antibody [N3C3] used in Western Blot (WB). GTX101035
Anti-Transferrin antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101035
Anti-Transferrin antibody [N3C3] used in Western Blot (WB). GTX101035
Anti-Transferrin antibody [N3C3] used in Western Blot (WB). GTX101035

Cat. No. GTX101035

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Transferrin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , human plasma , mouse plasma

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.12 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Transferrin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952202

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transferrin , HEL-S-71p , PRO1557 , PRO2086 , TFQTL1

Cellular Localization

Secreted

Background

The protein encoded by this gene is a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds 1 ion of ferric iron. The function of this encoded protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. In addition to its function in iron transport, this protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter/allergins from serum. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Transferrin antibody [N3C3] used in Western Blot (WB). GTX101035

GTX101035 WB Image

Human plasma (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Transferrin antibody [N3C3] (GTX101035) diluted at 1:1000. The HRP-conjugated anti-FALSE was used to detect the primary antibody.

Anti-Transferrin antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101035

GTX101035 IHC-P Image

Transferrin antibody [N3C3] detects Transferrin protein at cytoplasm in human esophageal carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
Transferrin antibody [N3C3] (GTX101035) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Transferrin antibody [N3C3] used in Western Blot (WB). GTX101035

GTX101035 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Transferrin antibody [N3C3] (GTX101035) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Transferrin antibody [N3C3] used in Western Blot (WB). GTX101035

GTX101035 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Transferrin antibody [N3C3] (GTX101035) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

Application Reference

LT Tsai et al. Antioxidants (Basel) 2023; 12 (11) : 1931 Inhibition of Indoxyl Sulfate-Induced Reactive Oxygen Species-Related Ferroptosis Alleviates Renal Cell Injury In Vitro and Chronic Kidney Disease Progression In Vivo
Application : WB
Hart V et al. J Cancer Res Clin Oncol 2021; HER2-PI9 and HER2-I12: two novel and functionally active splice variants of the oncogene HER2 in breast cancer.
Application : WB
Reactivity : Human
Yu W et al. Aging (Albany NY) 2019; 11 (23) : 11186-11201 Active compounds of herbs ameliorate impaired cognition in APP/PS1 mouse model of Alzheimer's disease.
Application : WB, IHC-P
Reactivity : Mouse
Lu C et al. J Vis Exp 2012; (63) : e3791 Chemically-blocked antibody microarray for multiplexed high-throughput profiling of specific protein glycosylation in complex samples.
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169