GeneTex
United States (US)

Transferrin antibody [N3C3]

GTX101035_39904_20151111_IHC-P_19011506_952.jpg
GTX101035_39904_20151231_WB_M_plasma_19011506_691.jpg
GTX101035_39904_20151231_WB_H_plasma_19011506_584.jpg
GTX101035_WB_19011506_371.jpg

Cat No. GTX101035

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , human plasma , mouse plasma

Predict Reactivity

Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.52mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Transferrin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DKFZp781D0156 antibody, PRO1557 antibody, PRO2086 antibody, TF antibody, serotransferrin antibody, beta-1 metal-binding globulin antibody, siderophilin antibody, transferrin antibody

Cellular Localization

Secreted

Background

The protein encoded by this gene is a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds 1 ion of ferric iron. The function of this encoded protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. In addition to its function in iron transport, this protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter/allergins from serum. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101035_39904_20151111_IHC-P_19011506_952.jpg

GTX101035 IHC-P Image

Transferrin antibody [N3C3] detects Transferrin protein at cytoplasm in human esophageal carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
Transferrin antibody [N3C3] (GTX101035) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101035_39904_20151231_WB_M_plasma_19011506_691.jpg

GTX101035 WB Image

Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Transferrin antibody [N3C3] (GTX101035) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101035_39904_20151231_WB_H_plasma_19011506_584.jpg

GTX101035 WB Image

Human tissue extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Transferrin antibody [N3C3] (GTX101035) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101035_WB_19011506_371.jpg

GTX101035 WB Image

Sample (30 µg of whole cell lysate)
A: A431 (GTX27909)
B: H1299
C: HeLa
7.5% SDS PAGE
GTX101035 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf