GeneTex
United States (US)

Transgelin 2 antibody [N1C3]

GTX115082_41059_IFA_18121410_214.jpg
GTX115082_40261_20141121_WB_18121410_629.jpg
GTX115082_41059_20170417_IHC-P_M_1_18121410_240.jpg
GTX115082_40261_WB_M_brain_18121410_858.jpg
GTX115082_WB_18121410_455.jpg
GTX115082_40261_20150410_IFA_18121410_624.jpg
GTX115082_41059_20170417_IHC-P_M_2_18121410_812.jpg

Cat No. GTX115082

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

293T , A431 , A549 , HeLa , mouse brain

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.53 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Transgelin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Transgelin 2,Ha1756,Tagln2

Background

The protein encoded by this gene is a homolog of the protein transgelin, which is one of the earliest markers of differentiated smooth muscle. The function of this protein has not yet been determined. [provided by RefSeq]

Database

DATA IMAGES
GTX115082_41059_IFA_18121410_214.jpg

GTX115082 ICC/IF Image

Transgelin 2 antibody [N1C3] detects Transgelin 2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A549 cells were fixed in ice-cold MeOH for 5 min.
Green: Transgelin 2 protein stained by Transgelin 2 antibody [N1C3] (GTX115082) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX115082_40261_20141121_WB_18121410_629.jpg

GTX115082 WB Image

Transgelin 2 antibody detects Transgelin 2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Transgelin 2 antibody (GTX115082) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115082_41059_20170417_IHC-P_M_1_18121410_240.jpg

GTX115082 IHC-P Image

Transgelin 2 antibody [N1C3] detects Transgelin 2 protein at cytoplasm in mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Transgelin 2 antibody [N1C3] (GTX115082) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115082_40261_WB_M_brain_18121410_858.jpg

GTX115082 WB Image

Sample (50 µg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX115082 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115082_WB_18121410_455.jpg

GTX115082 WB Image

Sample (30 µg of whole cell lysate)
A: A549
B: HeLa
12% SDS PAGE
GTX115082 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115082_40261_20150410_IFA_18121410_624.jpg

GTX115082 ICC/IF Image

Transgelin 2 antibody [N1C3] detects Transgelin 2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Transgelin 2 protein stained by Transgelin 2 antibody [N1C3] (GTX115082) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.

GTX115082_41059_20170417_IHC-P_M_2_18121410_812.jpg

GTX115082 IHC-P Image

Transgelin 2 antibody [N1C3] detects Transgelin 2 protein at cytoplasm in mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
Transgelin 2 antibody [N1C3] (GTX115082) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169