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Transgelin antibody, C-term

Anti-Transgelin antibody, C-term used in Western Blot (WB). GTX89789
Anti-Transgelin antibody, C-term used in Western Blot (WB). GTX89789

Cat. No. GTX89789

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all Transgelin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.01-0.03μg/ml
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89789-PEP).

Predict Reactivity

Rat, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-MTGYGRPRQIIS, from the C Terminus of the protein sequence according to NP_003177.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transgelin , SM22 , SM22-alpha , SMCC , TAGLN1 , WS3-10

Cellular Localization

Cytoplasm

Background

This gene encodes a shape change and transformation sensitive actin-binding protein which belongs to the calponin family. It is ubiquitously expressed in vascular and visceral smooth muscle, and is an early marker of smooth muscle differentiation. The encoded protein is thought to be involved in calcium-independent smooth muscle contraction. It acts as a tumor suppressor, and the loss of its expression is an early event in cell transformation and the development of some tumors, coinciding with cellular plasticity. The encoded protein has a domain architecture consisting of an N-terminal calponin homology (CH) domain and a C-terminal calponin-like (CLIK) domain. Mice with a knockout of the orthologous gene are viable and fertile but their vascular smooth muscle cells exhibit alterations in the distribution of the actin filament and changes in cytoskeletal organization. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-Transgelin antibody, C-term used in Western Blot (WB). GTX89789

GTX89789 WB Image

WB analysis of mouse skeletal muscle tissue lysate using GTX33555 Transgelin antibody.
Dilution : 0.03μg/ml
Loading : 35μg per lane

Anti-Transgelin antibody, C-term used in Western Blot (WB). GTX89789

GTX89789 WB Image

WB analysis of human skeletal muscle tissue lysate (A) and human testis tissue lysate (B) using GTX89789 Transgelin antibody, C-term.
Dilution : Lane A : 0.03μg/ml
Lane B : 0.003μg/ml
Loading : 35μg per lane

REFERENCE

Application Reference

Missinato MA et al. Nat Commun 2023; 14 (1) : 1709 Conserved transcription factors promote cell fate stability and restrict reprogramming potential in differentiated cells.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399