APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2
Predict Reactivity
Bovine, Chicken, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Transgelin. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888096
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
transgelin , SM22 , SM22-alpha , SMCC , TAGLN1 , WS3-10
Cellular Localization
Cytoplasm
Background
The protein encoded by this gene is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-Transgelin antibody [GT4511] used in Western Blot (WB). GTX629078](/templates/default/js/lightbox/images/loading.gif "Anti-Transgelin antibody [GT4511] used in Western Blot (WB). GTX629078")
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GTX629078 WB Image
Non-transfected (–) and transfected (+) HeLa whole cell extracts (15 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Transgelin antibody [GT4511] (GTX629078) diluted at 1:1000.
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GTX629078 IHC-P Image
Transgelin antibody [GT4511] detects Transgelin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse prostate.Transgelin stained by Transgelin antibody [GT4511] (GTX629078) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX629078 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Transgelin antibody [GT4511] (GTX629078) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX629078 IHC-P Image
Transgelin antibody [GT4511] detects Transgelin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.Transgelin stained by Transgelin antibody [GT4511] (GTX629078) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
There are currently no references for Transgelin antibody [GT4511] (GTX629078). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Transgelin antibody [GT4511] (GTX629078). Be the first to share your experience with this product.
![Anti-Transgelin antibody [GT4511] used in Western Blot (WB). GTX629078](/upload/website/prouct_img/small/GTX629078/GTX629078_41295_20160818_WB_shRNA_watermark_w_23061202_586.webp "Anti-Transgelin antibody [GT4511] used in Western Blot (WB). GTX629078")
![Anti-Transgelin antibody [GT4511] used in IHC (Paraffin sections) (IHC-P). GTX629078](/upload/website/prouct_img/small/GTX629078/GTX629078_41295_20180511_IHC-P_M_w_23061202_881.webp "Anti-Transgelin antibody [GT4511] used in IHC (Paraffin sections) (IHC-P). GTX629078")
![Anti-Transgelin antibody [GT4511] used in Western Blot (WB). GTX629078](/upload/website/prouct_img/small/GTX629078/GTX629078_41295_20170901_WB_w_23061202_564.webp "Anti-Transgelin antibody [GT4511] used in Western Blot (WB). GTX629078")
![Anti-Transgelin antibody [GT4511] used in IHC (Paraffin sections) (IHC-P). GTX629078](/upload/website/prouct_img/small/GTX629078/GTX629078_41295_20180511_IHC-P_R_w_23061202_367.webp "Anti-Transgelin antibody [GT4511] used in IHC (Paraffin sections) (IHC-P). GTX629078")
![Anti-Transgelin antibody [GT4511] used in Western Blot (WB). GTX629078](/upload/website/prouct_img/normal/GTX629078/GTX629078_41295_20160818_WB_shRNA_watermark_w_23061202_586.webp "Anti-Transgelin antibody [GT4511] used in Western Blot (WB). GTX629078")
![Anti-Transgelin antibody [GT4511] used in IHC (Paraffin sections) (IHC-P). GTX629078](/upload/website/prouct_img/normal/GTX629078/GTX629078_41295_20180511_IHC-P_M_w_23061202_881.webp "Anti-Transgelin antibody [GT4511] used in IHC (Paraffin sections) (IHC-P). GTX629078")
![Anti-Transgelin antibody [GT4511] used in Western Blot (WB). GTX629078](/upload/website/prouct_img/normal/GTX629078/GTX629078_41295_20170901_WB_w_23061202_564.webp "Anti-Transgelin antibody [GT4511] used in Western Blot (WB). GTX629078")
![Anti-Transgelin antibody [GT4511] used in IHC (Paraffin sections) (IHC-P). GTX629078](/upload/website/prouct_img/normal/GTX629078/GTX629078_41295_20180511_IHC-P_R_w_23061202_367.webp "Anti-Transgelin antibody [GT4511] used in IHC (Paraffin sections) (IHC-P). GTX629078")