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Transgelin antibody [HL1270]

Recombinant antibody Orthogonal
Anti-Transgelin antibody [HL1270] used in IHC (Paraffin sections) (IHC-P). GTX636672
Anti-Transgelin antibody [HL1270] used in Western Blot (WB). GTX636672
Anti-Transgelin antibody [HL1270] used in IHC (Paraffin sections) (IHC-P). GTX636672
Anti-Transgelin antibody [HL1270] used in IHC (Paraffin sections) (IHC-P). GTX636672
Anti-Transgelin antibody [HL1270] used in IHC (Paraffin sections) (IHC-P). GTX636672
Anti-Transgelin antibody [HL1270] used in Western Blot (WB). GTX636672

Cat. No. GTX636672

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1270

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Transgelin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Predict Reactivity

Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Transgelin. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910040

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transgelin , SM22 , SM22-alpha , SMCC , TAGLN1 , WS3-10

Cellular Localization

Cytoplasm

Background

This gene encodes a shape change and transformation sensitive actin-binding protein which belongs to the calponin family. It is ubiquitously expressed in vascular and visceral smooth muscle, and is an early marker of smooth muscle differentiation. The encoded protein is thought to be involved in calcium-independent smooth muscle contraction. It acts as a tumor suppressor, and the loss of its expression is an early event in cell transformation and the development of some tumors, coinciding with cellular plasticity. The encoded protein has a domain architecture consisting of an N-terminal calponin homology (CH) domain and a C-terminal calponin-like (CLIK) domain. Mice with a knockout of the orthologous gene are viable and fertile but their vascular smooth muscle cells exhibit alterations in the distribution of the actin filament and changes in cytoskeletal organization. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-Transgelin antibody [HL1270] used in IHC (Paraffin sections) (IHC-P). GTX636672

GTX636672 IHC-P Image

Transgelin antibody [HL1270] detects Transgelin protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
Transgelin stained by Transgelin antibody [HL1270] (GTX636672) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Transgelin antibody [HL1270] used in Western Blot (WB). GTX636672

GTX636672 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Transgelin antibody [HL1270] (GTX636672) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Transgelin antibody [HL1270] used in IHC (Paraffin sections) (IHC-P). GTX636672

GTX636672 IHC-P Image

Transgelin antibody [HL1270] detects Transgelin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse smooth muscle.
Transgelin stained by Transgelin antibody [HL1270] (GTX636672) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Transgelin antibody [HL1270] used in IHC (Paraffin sections) (IHC-P). GTX636672

GTX636672 IHC-P Image

Transgelin antibody [HL1270] detects Transgelin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
Transgelin stained by Transgelin antibody [HL1270] (GTX636672) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Transgelin antibody [HL1270] used in IHC (Paraffin sections) (IHC-P). GTX636672

GTX636672 IHC-P Image

Transgelin antibody [HL1270] detects Transgelin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse smooth muscle.
Transgelin stained by Transgelin antibody [HL1270] (GTX636672) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Transgelin antibody [HL1270] used in Western Blot (WB). GTX636672

GTX636672 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Transgelin antibody [HL1270] (GTX636672) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations