APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| 1-2μg/ml for 30 minutes at RT |
| 1-2μg/10⁶ cells |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
Product Note
This MAb recognizes a 22kDa protein, identified as Transgelin, also designated SM22-alpha. It may cross-react with SM22-beta.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant full-length human transgelin (TAGLN) protein.
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
SM22 , SMCC , TAGLN , TAGLN1 , WS310 , transgelin , Transgelin , SM22 alpha , Tagln
Cellular Localization
Cytoplasm
Background
This gene encodes a shape change and transformation sensitive actin-binding protein which belongs to the calponin family. It is ubiquitously expressed in vascular and visceral smooth muscle, and is an early marker of smooth muscle differentiation. The encoded protein is thought to be involved in calcium-independent smooth muscle contraction. It acts as a tumor suppressor, and the loss of its expression is an early event in cell transformation and the development of some tumors, coinciding with cellular plasticity. The encoded protein has a domain architecture consisting of an N-terminal calponin homology (CH) domain and a C-terminal calponin-like (CLIK) domain. Mice with a knockout of the orthologous gene are viable and fertile but their vascular smooth muscle cells exhibit alterations in the distribution of the actin filament and changes in cytoskeletal organization. [provided by RefSeq, Aug 2017]
Database
Research Area
DATA IMAGES
![Anti-Transgelin antibody [SPM606] used in IHC (Paraffin sections) (IHC-P). GTX35142](/templates/default/js/lightbox/images/loading.gif "Anti-Transgelin antibody [SPM606] used in IHC (Paraffin sections) (IHC-P). GTX35142")
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GTX35142 IHC-P Image
IHC-P analysis of human colon carcinoma tissue using GTX35142 Transgelin antibody [SPM606].
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REFERENCE
There are currently no references for Transgelin antibody [SPM606] (GTX35142). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Transgelin antibody [SPM606] (GTX35142). Be the first to share your experience with this product.
![Anti-Transgelin antibody [SPM606] used in IHC (Paraffin sections) (IHC-P). GTX35142](/upload/website/prouct_img/small/GTX35142/GTX35142_20200115_IHC-P_506_w_23060801_286.webp "Anti-Transgelin antibody [SPM606] used in IHC (Paraffin sections) (IHC-P). GTX35142")
![Anti-Transgelin antibody [SPM606] used in IHC (Paraffin sections) (IHC-P). GTX35142](/upload/website/prouct_img/normal/GTX35142/GTX35142_20200115_IHC-P_506_w_23060801_286.webp "Anti-Transgelin antibody [SPM606] used in IHC (Paraffin sections) (IHC-P). GTX35142")