APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:2000 |
| 1:100-1:500 |
| 1:50-1:200 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Zebrafish, Rabbit, Sheep, Bovine, Dog, Chicken, Pig, Horse, Xenopus(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 150 mM NaCl, 50% glycerol
Preservative
0.02% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human TAGLN(Accession Q01995), corresponding to amino acid residues E170-S201.
Purification
Purified by antigen-affinity chromatography
From serum
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
SM22,SMCC,TAGLN,TAGLN1,WS310,transgelin,Transgelin,SM22 alpha,Tagln
Cellular Localization
Cytoplasm
Background
This gene encodes a shape change and transformation sensitive actin-binding protein which belongs to the calponin family. It is ubiquitously expressed in vascular and visceral smooth muscle, and is an early marker of smooth muscle differentiation. The encoded protein is thought to be involved in calcium-independent smooth muscle contraction. It acts as a tumor suppressor, and the loss of its expression is an early event in cell transformation and the development of some tumors, coinciding with cellular plasticity. The encoded protein has a domain architecture consisting of an N-terminal calponin homology (CH) domain and a C-terminal calponin-like (CLIK) domain. Mice with a knockout of the orthologous gene are viable and fertile but their vascular smooth muscle cells exhibit alterations in the distribution of the actin filament and changes in cytoskeletal organization. [provided by RefSeq, Aug 2017]
Database
Research Area
DATA IMAGES
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GTX04760 IHC-P Image
IHC-P analysis of human ovary cancer tissue using GTX04760 Transgelin antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04760 IHC-P Image
IHC-P analysis of human ovary cancer and adjacent normal tissue using GTX04760 Transgelin antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04760 IHC-P Image
IHC-P analysis of human kidney cancer and adjacent normal tissue using GTX04760 Transgelin antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04760 IHC-P Image
IHC-P analysis of mouse testis tissue using GTX04760 Transgelin antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04760 IHC-P Image
IHC-P analysis of human gastric cancer tissue using GTX04760 Transgelin antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04760 IHC-P Image
IHC-P analysis of human mammary cancer tissue using GTX04760 Transgelin antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04760 IHC-P Image
IHC-P analysis of rat testis tissue using GTX04760 Transgelin antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04760 IHC-P Image
IHC-P analysis of human colorectal cancer tissue using GTX04760 Transgelin antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04760 IHC-P Image
IHC-P analysis of human pancreatic cancer tissue using GTX04760 Transgelin antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04760 IHC-P Image
IHC-P analysis of human lung cancer tissue using GTX04760 Transgelin antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04760 WB Image
WB analysis of 293T whole cell lysate using GTX04760 Transgelin antibody. The lane on the left was treated with blocking peptide.
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GTX04760 WB Image
WB analysis of mouse muscle tissue lysates using GTX04760 Transgelin antibody. The lane on right was treated with blocking peptide.
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GTX04760 IHC-P Image
IHC-P analysis of human prostate cancer tissue using GTX04760 Transgelin antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04760 WB Image
WB analysis of heat-shocked treated HeLa whole cell lysate using GTX04760 Transgelin antibody. The lane on the left was treated with blocking peptide.
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GTX04760 IHC-P Image
IHC-P analysis of rat ovary tissue using GTX04760 Transgelin antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04760 IHC-P Image
IHC-P analysis of mouse kidney tissue using GTX04760 Transgelin antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04760 IHC-P Image
IHC-P analysis of human gastric cancer tissue using GTX04760 Transgelin antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04760 ICC/IF Image
ICC/IF analysis of PFA-fixed A549 cells using GTX04760 Transgelin antibody.
Red : Primary antibody
Green : Beta tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100
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GTX04760 IHC-P Image
IHC-P analysis of rat stomach tissue using GTX04760 Transgelin antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04760 IHC-P Image
IHC-P analysis of human lung cancer tissue using GTX04760 Transgelin antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04760 IHC-P Image
IHC-P analysis of human gastric cancer and adjacent normal tissue using GTX04760 Transgelin antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04760 IHC-P Image
IHC-P analysis of human esophageal cancer tissue using GTX04760 Transgelin antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04760 IHC-P Image
IHC-P analysis of human colorectal cancer and adjacent normal tissue using GTX04760 Transgelin antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04760 IHC-P Image
IHC-P analysis of human esophageal cancer tissue using GTX04760 Transgelin antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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REFERENCE
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REVIEW
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