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Transgelin antibody

Anti-Transgelin antibody used in IHC (Paraffin sections) (IHC-P). GTX04763
Anti-Transgelin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04763
Anti-Transgelin antibody used in Flow cytometry (FACS). GTX04763
Anti-Transgelin antibody used in IHC (Paraffin sections) (IHC-P). GTX04763
Anti-Transgelin antibody used in IHC (Paraffin sections) (IHC-P). GTX04763
Anti-Transgelin antibody used in IHC (Paraffin sections) (IHC-P). GTX04763
Anti-Transgelin antibody used in IHC (Paraffin sections) (IHC-P). GTX04763
Anti-Transgelin antibody used in Western Blot (WB). GTX04763
Anti-Transgelin antibody used in IHC (Paraffin sections) (IHC-P). GTX04763
Anti-Transgelin antibody used in IHC (Paraffin sections) (IHC-P). GTX04763
Anti-Transgelin antibody used in IHC (Paraffin sections) (IHC-P). GTX04763

Cat. No. GTX04763

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($469)
See all Transgelin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.25-0.5 μg/mL
ICC/IF 5 μg/mL
IHC-P 2-5 μg/mL
FACS 1-3 μg/10⁶ cells
ELISA 0.1-0.5 μg/mL
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄

Preservative

No preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E.coli-derived human TAGLN/Transgelin recombinant protein (Position: Q15-E170).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SM22,SMCC,TAGLN,TAGLN1,WS310,transgelin,Transgelin,SM22 alpha,Tagln

Cellular Localization

Cytoplasm

Background

This gene encodes a shape change and transformation sensitive actin-binding protein which belongs to the calponin family. It is ubiquitously expressed in vascular and visceral smooth muscle, and is an early marker of smooth muscle differentiation. The encoded protein is thought to be involved in calcium-independent smooth muscle contraction. It acts as a tumor suppressor, and the loss of its expression is an early event in cell transformation and the development of some tumors, coinciding with cellular plasticity. The encoded protein has a domain architecture consisting of an N-terminal calponin homology (CH) domain and a C-terminal calponin-like (CLIK) domain. Mice with a knockout of the orthologous gene are viable and fertile but their vascular smooth muscle cells exhibit alterations in the distribution of the actin filament and changes in cytoskeletal organization. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-Transgelin antibody used in IHC (Paraffin sections) (IHC-P). GTX04763

GTX04763 IHC-P Image

IHC-P analysis of human adrenal cortical adenocarcinoma tissue using GTX04763 Transgelin antibody.
Antigen retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 2 μg/mL

Anti-Transgelin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04763

GTX04763 ICC/IF Image

ICC/IF analysis of Caco-2 cells using GTX04763 Transgelin antibody.
Antigen retrieval : Enzyme antigen retrieval was performed.
Green : Primary antibody
Blue : DAPI
Dilution : 5 μg/mL

Anti-Transgelin antibody used in Flow cytometry (FACS). GTX04763

GTX04763 FACS Image

FACS analysis of HepG2 cells using GTX04763 Transgelin antibody.
Blue : Primary antibody
Green : Isotype control
Red : Unlabeled cells
Dilution : 1 μg/10⁶ cells

Anti-Transgelin antibody used in IHC (Paraffin sections) (IHC-P). GTX04763

GTX04763 IHC-P Image

IHC-P analysis of human esophagus cancer tissue using GTX04763 Transgelin antibody.
Green : Primary antibody
Blue : DAPI
Antigen retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 5 μg/mL

Anti-Transgelin antibody used in IHC (Paraffin sections) (IHC-P). GTX04763

GTX04763 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX04763 Transgelin antibody.
Green : Primary antibody
Blue : DAPI
Antigen retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 5 μg/mL

Anti-Transgelin antibody used in IHC (Paraffin sections) (IHC-P). GTX04763

GTX04763 IHC-P Image

IHC-P analysis of rat heart tissue using GTX04763 Transgelin antibody.
Green : Primary antibody
Blue : DAPI
Antigen retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 5 μg/mL

Anti-Transgelin antibody used in IHC (Paraffin sections) (IHC-P). GTX04763

GTX04763 IHC-P Image

IHC-P analysis of human esophageal squamous carcinoma tissue using GTX04763 Transgelin antibody.
Antigen retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 2 μg/mL

Anti-Transgelin antibody used in Western Blot (WB). GTX04763

GTX04763 WB Image

WB analysis of various samples using GTX04763 Transgelin antibody.
Lane 1 : HepG2 whole cell lysate
Lane 2 : Rat stomach tissue lysate
Lane 3 : Mouse spleen tissue lysate
Loading : 30 μg
Dilution : 0.5 μg/mL

Anti-Transgelin antibody used in IHC (Paraffin sections) (IHC-P). GTX04763

GTX04763 IHC-P Image

IHC-P analysis of rat testis tissue using GTX04763 Transgelin antibody.
Antigen retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 2 μg/mL

Anti-Transgelin antibody used in IHC (Paraffin sections) (IHC-P). GTX04763

GTX04763 IHC-P Image

IHC-P analysis of human lymphoma tissue using GTX04763 Transgelin antibody.
Antigen retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 2 μg/mL

Anti-Transgelin antibody used in IHC (Paraffin sections) (IHC-P). GTX04763

GTX04763 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX04763 Transgelin antibody.
Antigen retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 2 μg/mL

REFERENCE

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Package List Price ($)
$ 469