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Transgelin antibody

KO/KD
Anti-Transgelin antibody used in Western Blot (WB). GTX101608
Anti-Transgelin antibody used in IHC (Paraffin sections) (IHC-P). GTX101608
Anti-Transgelin antibody used in Western Blot (WB). GTX101608
Anti-Transgelin antibody used in Immunoprecipitation (IP). GTX101608
Anti-Transgelin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101608
Anti-Transgelin antibody used in Western Blot (WB). GTX101608
Anti-Transgelin antibody used in Western Blot (WB). GTX101608
Anti-Transgelin antibody used in IHC (Paraffin sections) (IHC-P). GTX101608

Cat No. GTX101608

Reference 7

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)
See all Transgelin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , C8D30 , Rat-2

Predict Reactivity

Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.22 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Transgelin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952134

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

transgelin , SM22 , SM22-alpha , SMCC , TAGLN1 , WS3-10

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Transgelin antibody used in Western Blot (WB). GTX101608

GTX101608 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (15 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Transgelin antibody (GTX101608) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Transgelin antibody used in IHC (Paraffin sections) (IHC-P). GTX101608

GTX101608 IHC-P Image

Transgelin antibody detects Transgelin protein at cytoplasm in rat stomach by immunohistochemical analysis.
Sample: Paraffin-embedded rat stomach.
Transgelin antibody (GTX101608) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Transgelin antibody used in Western Blot (WB). GTX101608

GTX101608 WB Image

Transgelin antibody detects TAGLN protein by western blot analysis.
A. 30 μg Rat-2 whole cell lysate/extract
12% SDS-PAGE
Transgelin antibody (GTX101608) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Transgelin antibody used in Immunoprecipitation (IP). GTX101608

GTX101608 IP Image

Immunoprecipitation of Transgelin protein from HeLa whole cell extracts using 5 μg of Transgelin antibody (GTX101608).
Western blot analysis was performed using Transgelin antibody (GTX101608).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Transgelin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101608

GTX101608 ICC/IF Image

Transgelin antibody detects Transgelin protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Transgelin protein stained by Transgelin antibody (GTX101608) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-Transgelin antibody used in Western Blot (WB). GTX101608

GTX101608 WB Image

Transgelin antibody detects TAGLN protein by western blot analysis.
A. 30 μg C8D30 whole cell lysate/extract
12% SDS-PAGE
Transgelin antibody (GTX101608) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Transgelin antibody used in Western Blot (WB). GTX101608

GTX101608 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Transgelin antibody (GTX101608) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Transgelin antibody used in IHC (Paraffin sections) (IHC-P). GTX101608

GTX101608 IHC-P Image

Transgelin antibody detects Transgelin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse smooth muscle.
Transgelin stained by Transgelin antibody (GTX101608) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Andrew H Smith et al. JVS Vasc Sci 2020; 1 : 136-150 Canonical transient receptor potential 6 channel deficiency promotes smooth muscle cells dedifferentiation and increased proliferation after arterial injury.
Stratman AN et al. Commun Biol 2020; 3 (1) : 734 Chemokine mediated signalling within arteries promotes vascular smooth muscle cell recruitment.
Application : ICC/IF
Reactivity : Mouse
Hao W et al. Life Sci 2017; (Epub) Stellate ganglion block ameliorates vascular calcification by inhibiting endoplasmic reticulum stress.
Application : WB
Reactivity : Rat
Gautam J et al. Sci Rep 2016; 6 : 36450 The role of pericytic laminin in blood brain barrier integrity maintenance.
Application : ICC/IF
Reactivity : Mouse
Morita T et al. J Vet Med Sci 2014; 76 (7) : 963-9 Mechanisms underlying a decrease in KCl-induced contraction after long-term serum-free organ culture of rat isolated mesenteric artery.
Application : WB
Reactivity : Rat
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SDS
PBS.pdf
Package List Price ($)
$ 379
$ 169