Transgelin antibody

GTX101608_40058_20160818_WB_shRNA_watermark_19011506_835.jpg

Cat No. GTX101608

Reference 5

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse, Rat

See all Transgelin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , C8D30 , Rat-2

Predict Reactivity

Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.96 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Transgelin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Transgelin,Sm22,Sm22-Alpha,Smcc,Tagln1,Ws3-10,Tagln

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Gene ID: 6876
UniProt: Q01995

Research Area

DATA IMAGES

GTX101608 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (15 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Transgelin antibody (GTX101608) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101608 IHC-P Image

Transgelin antibody detects Transgelin protein at cytoplasm in rat stomach by immunohistochemical analysis.
Sample: Paraffin-embedded rat stomach.
Transgelin antibody (GTX101608) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101608 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Transgelin antibody (GTX101608) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101608 WB Image

Transgelin antibody detects TAGLN protein by western blot analysis.
A. 30 µg Rat-2 whole cell lysate/extract
12% SDS-PAGE
Transgelin antibody (GTX101608) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101608 WB Image

GTX101608 IP Image

Immunoprecipitation of Transgelin protein from HeLa whole cell extracts using 5 μg of Transgelin antibody (GTX101608).
Western blot analysis was performed using Transgelin antibody (GTX101608).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX101608 ICC/IF Image

Transgelin antibody detects Transgelin protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Transgelin protein stained by Transgelin antibody (GTX101608) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

GTX101608 WB Image

Transgelin antibody detects TAGLN protein by western blot analysis.
A. 30 µg C8D30 whole cell lysate/extract
12% SDS-PAGE
Transgelin antibody (GTX101608) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE