Transgelin antibody

Cat. No. GTX101608

Reference 8

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all Transgelin products

View product citations for antibody GTX101608 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

C8D30 , Rat-2 , HeLa

Predict Reactivity

Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.47 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Transgelin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952134

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transgelin , SM22 , SM22-alpha , SMCC , TAGLN1 , WS3-10

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Gene ID: 6876 TAGLN
UniProt: Q01995 TAGLN

Research Area

DATA IMAGES

GTX101608 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (15 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Transgelin antibody (GTX101608) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101608 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Transgelin antibody (GTX101608) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101608 WB Image

GTX101608 IHC-P Image

Transgelin antibody detects Transgelin protein at cytoplasm in rat stomach by immunohistochemical analysis.
Sample: Paraffin-embedded rat stomach.
Transgelin antibody (GTX101608) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101608 WB Image

Transgelin antibody detects TAGLN protein by western blot analysis.
A. 30 μg C8D30 whole cell lysate/extract
12% SDS-PAGE
Transgelin antibody (GTX101608) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101608 IHC-P Image

Transgelin antibody detects Transgelin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse smooth muscle.
Transgelin stained by Transgelin antibody (GTX101608) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101608 ICC/IF Image

Transgelin antibody detects Transgelin protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Transgelin protein stained by Transgelin antibody (GTX101608) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

GTX101608 IP Image

Immunoprecipitation of Transgelin protein from HeLa whole cell extracts using 5 μg of Transgelin antibody (GTX101608).
Western blot analysis was performed using Transgelin antibody (GTX101608).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX101608 WB Image

Transgelin antibody detects TAGLN protein by western blot analysis.
A. 30 μg Rat-2 whole cell lysate/extract
12% SDS-PAGE
Transgelin antibody (GTX101608) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.