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Transgelin antibody

Orthogonal
Anti-Transgelin antibody used in IHC (Paraffin sections) (IHC-P). GTX113561
Anti-Transgelin antibody used in Western Blot (WB). GTX113561
Anti-Transgelin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113561
Anti-Transgelin antibody used in IHC (Paraffin sections) (IHC-P). GTX113561
Anti-Transgelin antibody used in Western Blot (WB). GTX113561
Anti-Transgelin antibody used in Western Blot (WB). GTX113561
Anti-Transgelin antibody used in Western Blot (WB). GTX113561
Anti-Transgelin antibody used in IHC (Paraffin sections) (IHC-P). GTX113561
Anti-Transgelin antibody used in IHC (Frozen sections) (IHC-Fr). GTX113561
Anti-Transgelin antibody used in Western Blot (WB). GTX113561
Anti-Transgelin antibody used in Western Blot (WB). GTX113561

Cat. No. GTX113561

Reference 8
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat, Bovine
Package
100 μl ($399),
25 μl ($169)
See all Transgelin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

A-10 treated with MG132 , Mouse liver

Predict Reactivity

Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Transgelin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620222

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transgelin , SM22 , SM22-alpha , SMCC , TAGLN1 , WS3-10

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Transgelin antibody used in IHC (Paraffin sections) (IHC-P). GTX113561

GTX113561 IHC-P Image

Transgelin antibody detects Transgelin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse smooth muscle.
Transgelin stained by Transgelin antibody (GTX113561) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Transgelin antibody used in Western Blot (WB). GTX113561

GTX113561 WB Image

Sample (50 μg of whole cell lysate)
A: mouse liver
12% SDS PAGE
GTX113561 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Transgelin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113561

GTX113561 ICC/IF Image

Transgelin antibody detects Transgelin protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Transgelin protein stained by Transgelin antibody (GTX113561) diluted at 1:100.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-Transgelin antibody used in IHC (Paraffin sections) (IHC-P). GTX113561

GTX113561 IHC-P Image

Transgelin antibody detects Transgelin protein at cytosol on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
Transgelin antibody (GTX113561) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Transgelin antibody used in Western Blot (WB). GTX113561

GTX113561 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Transgelin antibody (GTX113561) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Transgelin antibody used in Western Blot (WB). GTX113561

GTX113561 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Transgelin antibody (GTX113561) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Transgelin antibody used in Western Blot (WB). GTX113561

GTX113561 WB Image

Western blot analysis of Transgelin (GTX113561, upper panel) and beta-actin (lower panel) 
Sample (35 μg of whole cell lysate) 
A: A-10 cell
B: A-10 cell treated with MG132
GTX113561 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Transgelin antibody used in IHC (Paraffin sections) (IHC-P). GTX113561

GTX113561 IHC-P Image

Transgelin antibody detects Transgelin protein at cytosol on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Transgelin antibody (GTX113561) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Transgelin antibody used in IHC (Frozen sections) (IHC-Fr). GTX113561

GTX113561 IHC-Fr Image

The data was published in the journal Sci Rep in 2016. PMID: 27808256

Anti-Transgelin antibody used in Western Blot (WB). GTX113561

GTX113561 WB Image

The data was published in the journal Nat Commun in 2014. PMID: 24583950

Anti-Transgelin antibody used in Western Blot (WB). GTX113561

GTX113561 WB Image

The data was published in the journal Nat Commun in 2014. PMID: 24583950

REFERENCE

Application Reference

Delve E et al. Osteoarthr Cartil Open 2020; 2 (3) : 100071 Superficial and deep zone articular chondrocytes exhibit differences in actin polymerization status and actin-associated molecules in vitro.
Gautam J et al. Sci Rep 2016; 6 : 36450 The role of pericytic laminin in blood brain barrier integrity maintenance.
Yao Y et al. Nat Commun 2016; 7 : 11415 Laminin regulates PDGFRβ(+) cell stemness and muscle development.
Application : WB, IHC-Fr
Reactivity : Mouse
Parreno J et al. Matrix Biol 2016; (Epub) MRTF-A signaling regulates the acquisition of the contractile phenotype in dedifferentiated chondrocytes.
Application : WB
Reactivity : Bovine
Tang D et al. BMC Genomics 2016; 17 : 488 Integrated analysis of mRNA, microRNA and protein in systemic lupus erythematosus-specific induced pluripotent stem cells from urine.
Application : WB
Reactivity : Human
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Submit a Reference

REVIEW

Transgelin antibody Cat. No. GTX113561

Rating
( Average 4.7 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 4 based on 1 users reviews)
Anti-Transgelin antibody used in IHC (Paraffin sections) (IHC-P). GTX113561
Date : Anonymous submitted on 25-Apr-2018
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : liver tissue
Blocking : SuperBlockTM Blocking Buffer in PBS, 25°C, 0.5Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : Dako Target Retrieval Solution
Primary Antibody : 1:500, 4°C, 18Hr
Figure Note : Mouse liver treated CCl4 after 2 days.
Anti-Transgelin antibody used in Western Blot (WB). GTX113561
Date : Anonymous submitted on 05-Mar-2013
Score :
Application Tested : WB
Sample Species : Bov
Sample : Fibroblastic Chondrocyte tissue
Amount used : 30μg
Blocking : 5% skim milk in PBS, 24°C, 0.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-Transgelin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113561
Date : Anonymous submitted on 25-Jan-2013
Score :
Application Tested : ICC/IF
Sample Species : Bov
Sample : smooth muscle cells
Blocking : 10% goat serum, 24°C, 0.5Hr
Fixation : 4% paraformaldehyde, 24°C, 0.17Hr
Primary Antibody : 1:100, 4°C, 16Hr
Figure Note : Counterstained with Phalloidin-598 Conjugate for actin and DAPI for Nucleus
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169