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Transglutaminase 2 antibody [SPM592]

Anti-Transglutaminase 2 antibody [SPM592] used in IHC (Paraffin sections) (IHC-P). GTX35144

Cat. No. GTX35144

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 SPM592

Isotype

 IgG2a

Application

 ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat, Rabbit, Monkey
Package
100 μg ($529)
See all Transglutaminase 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml|30 min at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human TGM2 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TG(C) , TGC , TGM2 , transglutaminase 2 , Transglutaminase 2 , Tissue transglutaminase

Background

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Transglutaminase 2 antibody [SPM592] used in IHC (Paraffin sections) (IHC-P). GTX35144

GTX35144 IHC-P Image

IHC-P analysis of human placenta tissue using GTX35144 Transglutaminase 2 antibody [SPM592].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529