GeneTex
United States (US)

Transglutaminase 2 antibody [TGM2/419]

GTX35143_20170712_IHC-P_1_18121410_914.jpg
GTX35143_20170712_IHC-P_2_18121410_674.jpg
GTX35143_20170712_IHC-P_18121410_202.jpg
GTX35143_20170712_IHC-P_3_18121410_909.jpg

Cat No. GTX35143

Host Mouse
Clonality Monoclonal
Clone Name TGM2/419
Isotype IgG2a
Application ICC/IF, IHC-P
Reactivity Human, Mouse, Rat, Rabbit, Monkey
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 0.5-1ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Specificity/Sensitivity

Recognizes a 77-85kDa protein, identified as cellular or tissue transglutaminase II (TGase II). Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. The identification of transglutaminase as the main antigen of endomysium antibodies allows a new diagnostic approach to celiac disease (CD), a genetic, immunologically mediated small bowel enteropathy that causes malabsorption. TGase II is implicated in programmed cell death, signal transduction, drug-resistance, cell growth, endocytosis, insulin secretion, cell adhesion, cataract formation, and wound healing.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant full-length human TGM2 protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Transglutaminase 2,Tg(C),Tgc,Tgm2

Background

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX35143_20170712_IHC-P_1_18121410_914.jpg

GTX35143 IHC-P Image

Formalin-fixed, paraffin-embedded human Placenta stained with Transglutaminase II Monoclonal Antibody (TGM2/419)

GTX35143_20170712_IHC-P_2_18121410_674.jpg

GTX35143 IHC-P Image

Formalin-fixed, paraffin-embedded human Uterus stained with Transglutaminase II Monoclonal Antibody (TGM2/419)

GTX35143_20170712_IHC-P_18121410_202.jpg

GTX35143 IHC-P Image

Formalin-fixed, paraffin-embedded human Tonsil stained with Transglutaminase II Monoclonal Antibody (TGM2/419)

GTX35143_20170712_IHC-P_3_18121410_909.jpg

GTX35143 IHC-P Image

Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Transglutaminase II Monoclonal Antibody (TGM2/419)