Transketolase antibody [N3C2], Internal

Name: Transketolase antibody [N3C2], Internal
Brand: GeneTex
SKU: GTX101477
Price: 399.00 USD
Rating: 3 (1 reviews)

Anti-Transketolase antibody [N3C2], Internal used in Western Blot (WB). GTX101477

Anti-Transketolase antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX101477

Anti-Transketolase antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101477

Cat. No. GTX101477

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all Transketolase products

Datasheet
Document

View product citations for antibody GTX101477 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

Raji , 293T , A431 , HeLa , HepG2 , mouse liver

Predict Reactivity

Rat, Bovine, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Transketolase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168757

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transketolase , HEL-S-48 , HEL107 , SDDHD , TK , TKT1

Background

This gene encodes a thiamine-dependent enzyme which plays a role in the channeling of excess sugar phosphates to glycolysis in the pentose phosphate pathway. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Database

Gene ID: 7086 TKT
UniProt: P29401 TKT

Research Area

DATA IMAGES

GTX101477 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Transketolase antibody [N3C2], Internal (GTX101477) diluted at 1:1000.

GTX101477 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
7.5% SDS PAGE
GTX101477 diluted at 1:1000

GTX101477 IHC-P Image

Transketolase antibody [N3C2], Internal detects Transketolase protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Transketolase antibody [N3C2], Internal (GTX101477) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101477 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Transketolase antibody [N3C2], Internal (GTX101477) diluted at 1:1000.

GTX101477 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using Transketolase(GTX101477) antibody at 1:500 dilution.

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REVIEW