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Transketolase antibody [N3C2], Internal

Anti-Transketolase antibody [N3C2], Internal used in Western Blot (WB). GTX101477
Anti-Transketolase antibody [N3C2], Internal used in Western Blot (WB). GTX101477
Anti-Transketolase antibody [N3C2], Internal used in Western Blot (WB). GTX101477
Anti-Transketolase antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101477
Anti-Transketolase antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX101477

Cat No. GTX101477

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

Raji , mouse liver , 293T , A431 , HeLa , HepG2 , *human endothelial cells

Predict Reactivity

Rat, Bovine, Pig, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Transketolase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

transketolase,HEL-S-48,HEL107,SDDHD,TK,TKT1

Background

This gene encodes a thiamine-dependent enzyme which plays a role in the channeling of excess sugar phosphates to glycolysis in the pentose phosphate pathway. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Transketolase antibody [N3C2], Internal used in Western Blot (WB). GTX101477

GTX101477 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
7.5% SDS PAGE
GTX101477 diluted at 1:1000

Anti-Transketolase antibody [N3C2], Internal used in Western Blot (WB). GTX101477

GTX101477 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Transketolase antibody [N3C2], Internal (GTX101477) diluted at 1:1000.

Anti-Transketolase antibody [N3C2], Internal used in Western Blot (WB). GTX101477

GTX101477 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Transketolase antibody [N3C2], Internal (GTX101477) diluted at 1:1000.

Anti-Transketolase antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101477

GTX101477 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using Transketolase(GTX101477) antibody at 1:500 dilution.

Anti-Transketolase antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX101477

GTX101477 IHC-P Image

Transketolase antibody [N3C2], Internal detects Transketolase protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Transketolase antibody [N3C2], Internal (GTX101477) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
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REVIEW

Transketolase antibody [N3C2], Internal Cat No. GTX101477

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 12-Nov-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : human endothelial cells
Amount used : 10μg
Blocking : 3% BSA in PBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 17Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169