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Trefoil Factor 3 antibody

Anti-Trefoil Factor 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX134098
Anti-Trefoil Factor 3 antibody used in Western Blot (WB). GTX134098
Anti-Trefoil Factor 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX134098

Cat No. GTX134098

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

10 kDa. ( Note )

Positive Control

MCF-7 , MDA-MB-231

Predict Reactivity

Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.19 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Trefoil Factor 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

trefoil factor 3,ITF,P1B,TFI

Cellular Localization

Cytoplasm

Background

Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
Anti-Trefoil Factor 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX134098

GTX134098 IHC-P Image

Trefoil Factor 3 antibody detects Trefoil Factor 3 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Trefoil Factor 3 stained by Trefoil Factor 3 antibody (GTX134098) diluted at 1:1500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Trefoil Factor 3 antibody used in Western Blot (WB). GTX134098

GTX134098 WB Image

Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Trefoil Factor 3 antibody (GTX134098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Trefoil Factor 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX134098

GTX134098 IHC-P Image

Trefoil Factor 3 antibody detects secreted Trefoil Factor 3 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Trefoil Factor 3 stained by Trefoil Factor 3 antibody (GTX134098) diluted at 1:1500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169