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Trefoil Factor 3 antibody

Anti-Trefoil Factor 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31422
Anti-Trefoil Factor 3 antibody used in Western Blot (WB). GTX31422
Anti-Trefoil Factor 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX31422
Anti-Trefoil Factor 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX31422
Anti-Trefoil Factor 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX31422

Cat No. GTX31422

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
ICC/IF 2 μg/mL
IHC-P 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

10 kDa. ( Note )

Positive Control

Human Colon Tissue Lysate(GTX27928) , Human Small Intestine Tissue Slide

Specificity/Sensitivity

TFF3 antibody is predicted to not cross-react with other TFF family members.

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Trefoil Factor 3 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human Trefoil Factor 3.The immunogen is located within the last 50 amino acids of Trefoil Factor 3.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

trefoil factor 3 , ITF , P1B , TFI

Cellular Localization

Cytoplasm

Background

Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Trefoil Factor 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31422

GTX31422 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31422 Trefoil Factor 3 antibody.
Working concentration : 2 μg/ml

Anti-Trefoil Factor 3 antibody used in Western Blot (WB). GTX31422

GTX31422 WB Image

WB analysis of human colon tissue lysate in (A) the absence and (B) the presence of blocking peptide using GTX31422 Trefoil Factor 3 antibody.
Working concentration : 1 μg/ml

Anti-Trefoil Factor 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX31422

GTX31422 IHC-P Image

IHC-P analysis of human small intestine tissue using GTX31422 Trefoil Factor 3 antibody.
Working concentration : 2 μg/ml

Anti-Trefoil Factor 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX31422

GTX31422 IHC-P Image

IHC-P analysis of human small intestine tissue using GTX31422 Trefoil Factor 3 antibody.
Working concentration : 2.5 μg/ml

Anti-Trefoil Factor 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX31422

GTX31422 IHC-P Image

IHC-P analysis of human small intestine tissue using GTX31422 Trefoil Factor 3 antibody.
Working concentration : 20 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339