GeneTex
United States (US)

Trident Blue Prestained Protein Ladder

GTX16376_WB_1_18121410_853.jpg

Cat No. GTX16376

Application WB
APPLICATION

Application Note

˙3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
˙1.5~2.5 μl per well for general Western transferring.
˙Apply more for thicker (> 1.5 mm) or larger gel.
PROPERTIES

Form

Liquid

Storage

Stable for up to 2 weeks at 25ºCStable for up to 3 months at 4ºC. For long term storage, store at -20ºC

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

The Trident Blue Prestained Protein Ladder is a blue protein standard with 11 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa respectively) are enhanced in intensity when separated on SDS-PAGE (Tris-glycine buffer). The Trident Blue Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.
DATA IMAGES
GTX16376_WB_1_18121410_853.jpg

GTX16376 WB Image

Migration patterns of Trident Blue Prestained Protein Ladder in different electrophoresis conditions

Package List Price ($)
$ 119