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Trident Prestained Protein Ladder (Standard Range)

Trident Prestained Protein Ladder (Standard Range). GTX49384
Trident Prestained Protein Ladder (Standard Range). GTX49384

Cat No. GTX49384

Application

WB
Package
500 μl ($99)
PRODUCT

Summary

The GTX49384 Trident Prestained Protein Ladder (Standard Range) is a ready-to-use three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine Buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and 10 to 180 kDa Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-Glycine buffer). GTX49384 Trident Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

APPLICATION

Application Note

Under suggested conditions, Trident Prestained Protein Ladder resolves 10 major bands in 15% SDS-PAGE (Tris-Glycine buffer) and after Western blotting to nitrocellulose membrane. 5 μl per loading for clear visualization during electrophoresis on a 15-well or 10-well mini-gel.

PROPERTIES

Form

Liquid

Buffer

20 mM Tris-phosphate pH 7.5, 2% SDS, 0.2 mM DTT, 3.6 M urea, 15% (v/v) glycerol

Storage

For short-term storage, store at 4ºC (up to 3 months). For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1~0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

DATA IMAGES

Trident Prestained Protein Ladder (Standard Range). GTX49384

GTX49384 Image

Migration pattern of GTX49384 Trident Prestained Protein Ladder (Standard Range) in Tris-Glycine gel.

Trident Prestained Protein Ladder (Standard Range). GTX49384

GTX49384 Image

Migration pattern of GTX49384 Trident Prestained Protein Ladder (Standard Range) in Bis-Tris- gel.

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Package List Price ($)
$ 99