GeneTex
United States (US)

Trident pico Western HRP Substrate

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Cat No. GTX17435

Application WB
PRODUCT

Content

Trident femto-ECL Content Package
GTX17435 (100ml) GTX17435 Trident pico Western HRP Substrate Solution A 50ml
GTX17435 Trident pico Western HRP Substrate Solution B 50ml
GTX17435 (500ml) GTX17435 Trident pico Western HRP Substrate Solution A 250ml
GTX17435 Trident pico Western HRP Substrate Solution B 250ml

 

PROPERTIES

Form

Liquid

Storage

Trident pico Western HRP Substrate shelf life is 24 months when stored in the dark at 2ºC to 8ºC. Keep container tightly closed. Store Trident pico Western HRP Substrate away from heat or light. Mixed substrate can be stored for up to 24 hours at 2-8ºC.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Trident pico-ECL

Background

Trident pico Western HRP Substrate can detect horseradish peroxidase at the low pico to mid femtogram level. Trident pico Western HRP Substrate provides superior sensitivity compared to competitor products and is more economical. The substrate is supplied as two components. Substrate may be used for any blotting application utilizing horseradish peroxidase (HRP)-conjugates. The substrate can be used with various blocking buffers and on nitrocellulose or PVDF membranes. Detection and analysis may be done by CCD imaging systems or x-ray film.
DATA IMAGES
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GTX17435 WB Image

Serial dilutions of purified mCherry recombinant protein were prepared and applied at 10 μL/well. After electrophoresis and protein transfer, membranes were probed with anti-mCherry antibody (GTX128508) diluted at 1:2000 and then with goat anti-rabbit HRP-conjugated secondary antibody (1:2000). Identical blots were incubated with 1 ml of Trident femto Western HRP Substrate, SuperSignalTM West Femto, Trident pico Western HRP Substrate, ClarityTM, Trident plus Western HRP Substrate, and LuminataTM Forte. The blots were simultaneously exposed for 36 seconds and 180 seconds using BioSpectrumTM 500 digital imaging system. SuperSignalTM West Femto, ClarityTM, LuminataTM Forte are registered trademark of Thermo Fisher Scientific, Bio-Rad, and EMD Millipore, respectively. The trademark holder is not affiliated with GeneTex and does not endorse this product.

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GTX17435 Image


Package List Price ($)
$ 249
$ 79