APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.01-0.03μg/ml |
| 5μg/ml |
|
Note :
IHC-P
Human Liver shows staining of the sinusoids.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89594-PEP).
Product Note
This antibody is expected to recognise reported isoforms 1 and 2 (NP_000356.1; NP_001152759.1).
Predict Reactivity
Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-LKPEFVDIINAKQ, from the C Terminus of the protein sequence according to NP_000356.1; NP_001152759.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
triosephosphate isomerase 1 , HEL-S-49 , TIM , TPI , TPID
Cellular Localization
Cytoplasm
Background
This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]
Database
Research Area
DATA IMAGES
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GTX89594 IHC-P Image
IHC-P analysis of human pancreas using GTX89594 Triosephosphate isomerase antibody, C-term.
Antigen retrieval : Tris/EDTA buffer pH 9
Dilution : 5μg/ml
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GTX89594 WB Image
WB analysis of human liver lysate using GTX89594 Triosephosphate isomerase antibody, C-term.
Dilution : 0.003μg/ml
Loading : 30μg protein in RIPA buffer
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GTX89594 IHC-P Image
IHC-P analysis of human liver using GTX89594 Triosephosphate isomerase antibody, C-term.
Antigen retrieval : Tris/EDTA buffer pH 9
Dilution : 2μg/ml
|
REFERENCE
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REVIEW
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