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Triosephosphate isomerase antibody [C2C3], C-term

Anti-Triosephosphate isomerase antibody [C2C3], C-term used in Western Blot (WB). GTX104618
Anti-Triosephosphate isomerase antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104618
Anti-Triosephosphate isomerase antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104618
Anti-Triosephosphate isomerase antibody [C2C3], C-term used in Western Blot (WB). GTX104618
Anti-Triosephosphate isomerase antibody [C2C3], C-term used in Western Blot (WB). GTX104618
Anti-Triosephosphate isomerase antibody [C2C3], C-term used in Western Blot (WB). GTX104618

Cat No. GTX104618

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, ELISA
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat2

Predict Reactivity

Bovine, Dog, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Triosephosphate isomerase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

triosephosphate isomerase 1 , HEL-S-49 , TIM , TPI , TPID

Background

This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Triosephosphate isomerase antibody [C2C3], C-term used in Western Blot (WB). GTX104618

GTX104618 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody [C2C3], C-term (GTX104618) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Triosephosphate isomerase antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104618

GTX104618 ICC/IF Image

Triosephosphate isomerase antibody [C2C3], C-term detects Triosephosphate isomerase protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Triosephosphate isomerase stained by Triosephosphate isomerase antibody [C2C3], C-term (GTX104618) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

Anti-Triosephosphate isomerase antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104618

GTX104618 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SNU-16 xenograft, using TPI1 (GTX104618) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Triosephosphate isomerase antibody [C2C3], C-term used in Western Blot (WB). GTX104618

GTX104618 WB Image

Triosephosphate isomerase antibody detects Triosephosphate isomerase protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody (GTX104618) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Triosephosphate isomerase antibody [C2C3], C-term used in Western Blot (WB). GTX104618

GTX104618 WB Image

Triosephosphate isomerase antibody detects Triosephosphate isomerase protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody (GTX104618) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Triosephosphate isomerase antibody [C2C3], C-term used in Western Blot (WB). GTX104618

GTX104618 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody [C2C3], C-term (GTX104618) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169