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Triosephosphate isomerase antibody [C2C3], C-term

KO/KD Orthogonal
Anti-Triosephosphate isomerase antibody [C2C3], C-term used in Western Blot (WB). GTX104618
Anti-Triosephosphate isomerase antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104618
Anti-Triosephosphate isomerase antibody [C2C3], C-term used in Western Blot (WB). GTX104618
Anti-Triosephosphate isomerase antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104618
Anti-Triosephosphate isomerase antibody [C2C3], C-term used in Western Blot (WB). GTX104618
Anti-Triosephosphate isomerase antibody [C2C3], C-term used in Western Blot (WB). GTX104618
Anti-Triosephosphate isomerase antibody [C2C3], C-term used in Western Blot (WB). GTX104618

Cat. No. GTX104618

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Triosephosphate isomerase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat2 , 293T , A431 , HeLa , HepG2

Predict Reactivity

Drosophila, Bovine, Dog, Chicken, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Triosephosphate isomerase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241405

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

triosephosphate isomerase 1 , HEL-S-49 , TIM , TPI , TPID

Background

This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Triosephosphate isomerase antibody [C2C3], C-term used in Western Blot (WB). GTX104618

GTX104618 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody [C2C3], C-term (GTX104618) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Triosephosphate isomerase antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104618

GTX104618 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SNU-16 xenograft, using TPI1 (GTX104618) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Triosephosphate isomerase antibody [C2C3], C-term used in Western Blot (WB). GTX104618

GTX104618 WB Image

Triosephosphate isomerase antibody detects Triosephosphate isomerase protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody (GTX104618) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Triosephosphate isomerase antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104618

GTX104618 ICC/IF Image

Triosephosphate isomerase antibody [C2C3], C-term detects Triosephosphate isomerase protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Triosephosphate isomerase stained by Triosephosphate isomerase antibody [C2C3], C-term (GTX104618) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

Anti-Triosephosphate isomerase antibody [C2C3], C-term used in Western Blot (WB). GTX104618

GTX104618 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody [C2C3], C-term (GTX104618) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Triosephosphate isomerase antibody [C2C3], C-term used in Western Blot (WB). GTX104618

GTX104618 WB Image

Triosephosphate isomerase antibody detects Triosephosphate isomerase protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody (GTX104618) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Triosephosphate isomerase antibody [C2C3], C-term used in Western Blot (WB). GTX104618

GTX104618 WB Image

Orthogonal

The data was published in the journal Evid Based Complement Alternat Med in 2012. PMID: 22536285

REFERENCE

Application Reference

Chen SM et al. PLoS One 2020; 15 (1) : e0227838 Effect of prednisolone on glyoxalase 1 in an inbred mouse model of aristolochic acid nephropathy using a proteomics method with fluorogenic derivatization-liquid chromatography-tandem mass spectrometry.
Ludvigsen M et al. Blood Adv 2018; 2 (19) : 2533-2542 Proteomic profiling identifies outcome-predictive markers in patients with peripheral T-cell lymphoma, not otherwise specified.
Application : IHC-P
Reactivity : Human
Chen JY et al. J Nutr Biochem 2013; 24 (11) : 1889-910 High glucose-induced proteome alterations in hepatocytes and its possible relevance to diabetic liver disease.
Application : ELISA
Reactivity : Human
Hsiao CY et al. Evid Based Complement Alternat Med 2012; 2012 : 467531 A Study of the Wound Healing Mechanism of a Traditional Chinese Medicine, Angelica sinensis, Using a Proteomic Approach.
Linge A et al. Invest Ophthalmol Vis Sci 2012; 53 (8) : 4634-43 Differential expression of fourteen proteins between uveal melanoma from patients who subsequently developed distant metastases versus those who did Not.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169