APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2
Predict Reactivity
Drosophila, Bovine, Dog, Chicken, Xenopus tropicalis, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Triosephosphate isomerase. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888233
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
triosephosphate isomerase 1 , HEL-S-49 , TIM , TPI , TPID
Background
This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-Triosephosphate isomerase antibody [GT181] used in Western Blot (WB). GTX630779](/templates/default/js/lightbox/images/loading.gif "Anti-Triosephosphate isomerase antibody [GT181] used in Western Blot (WB). GTX630779")
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GTX630779 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody [GT181] (GTX630779) diluted at 1:500.
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GTX630779 IHC-P Image
Triosephosphate isomerase antibody [GT181] detects Triosephosphate isomerase protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.Triosephosphate isomerase stained by Triosephosphate isomerase antibody [GT181] (GTX630779) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX630779 WB Image
Triosephosphate isomerase antibody [GT181] detects Triosephosphate isomerase protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg A431 whole cell extract
C. 30 μg HeLa whole cell extract
D. 30 μg HepG2 whole cell extract
12 % SDS-PAGE
Triosephosphate isomerase antibody [GT181] (GTX630779) dilution: 1:1000
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GTX630779 WB Image
Triosephosphate isomerase antibody [GT181] detects Triosephosphate isomerase protein by western blot analysis.
A. 30 μg PC-12 whole cell extract
B. 30 μg Rat2 whole cell extract
12 % SDS-PAGE
Triosephosphate isomerase antibody [GT181] (GTX630779) dilution: 1:1000
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GTX630779 WB Image
Triosephosphate isomerase antibody [GT181] detects Triosephosphate isomerase protein by western blot analysis.
A. 30 μg Neuro2A whole cell extract
B. 30 μg C8D30 whole cell extract
C. 30 μg NIH-3T3 whole cell extract
D. 30 μg Raw264.7 whole cell extract
E. 30 μg C2C12 whole cell extract
12 % SDS-PAGE
Triosephosphate isomerase antibody [GT181] (GTX630779) dilution: 1:1000
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GTX630779 ICC/IF Image
Triosephosphate isomerase antibody [GT181] detects Triosephosphate isomerase protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Triosephosphate isomerase protein stained by Triosephosphate isomerase antibody [GT181] (GTX630779) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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REFERENCE
There are currently no references for Triosephosphate isomerase antibody [GT181] (GTX630779). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Triosephosphate isomerase antibody [GT181] (GTX630779). Be the first to share your experience with this product.
![Anti-Triosephosphate isomerase antibody [GT181] used in Western Blot (WB). GTX630779](/upload/website/prouct_img/small/GTX630779/GTX630779_41631_20161020_WB_shRNA_watermark_w_23061202_165.webp "Anti-Triosephosphate isomerase antibody [GT181] used in Western Blot (WB). GTX630779")
![Anti-Triosephosphate isomerase antibody [GT181] used in IHC (Paraffin sections) (IHC-P). GTX630779](/upload/website/prouct_img/small/GTX630779/GTX630779_41631_20180511_IHC-P_M_w_23061202_541.webp "Anti-Triosephosphate isomerase antibody [GT181] used in IHC (Paraffin sections) (IHC-P). GTX630779")
![Anti-Triosephosphate isomerase antibody [GT181] used in Western Blot (WB). GTX630779](/upload/website/prouct_img/small/GTX630779/GTX630779_41631_WB_w_23061202_222.webp "Anti-Triosephosphate isomerase antibody [GT181] used in Western Blot (WB). GTX630779")
![Anti-Triosephosphate isomerase antibody [GT181] used in Western Blot (WB). GTX630779](/upload/website/prouct_img/small/GTX630779/GTX630779_41631_WB_R_w_23061202_757.webp "Anti-Triosephosphate isomerase antibody [GT181] used in Western Blot (WB). GTX630779")
![Anti-Triosephosphate isomerase antibody [GT181] used in Western Blot (WB). GTX630779](/upload/website/prouct_img/small/GTX630779/GTX630779_41631_WB_M_w_23061202_868.webp "Anti-Triosephosphate isomerase antibody [GT181] used in Western Blot (WB). GTX630779")
![Anti-Triosephosphate isomerase antibody [GT181] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630779](/upload/website/prouct_img/small/GTX630779/GTX630779_41631_IFA_w_23061202_123.webp "Anti-Triosephosphate isomerase antibody [GT181] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630779")
![Anti-Triosephosphate isomerase antibody [GT181] used in Western Blot (WB). GTX630779](/upload/website/prouct_img/normal/GTX630779/GTX630779_41631_20161020_WB_shRNA_watermark_w_23061202_165.webp "Anti-Triosephosphate isomerase antibody [GT181] used in Western Blot (WB). GTX630779")
![Anti-Triosephosphate isomerase antibody [GT181] used in IHC (Paraffin sections) (IHC-P). GTX630779](/upload/website/prouct_img/normal/GTX630779/GTX630779_41631_20180511_IHC-P_M_w_23061202_541.webp "Anti-Triosephosphate isomerase antibody [GT181] used in IHC (Paraffin sections) (IHC-P). GTX630779")
![Anti-Triosephosphate isomerase antibody [GT181] used in Western Blot (WB). GTX630779](/upload/website/prouct_img/normal/GTX630779/GTX630779_41631_WB_w_23061202_222.webp "Anti-Triosephosphate isomerase antibody [GT181] used in Western Blot (WB). GTX630779")
![Anti-Triosephosphate isomerase antibody [GT181] used in Western Blot (WB). GTX630779](/upload/website/prouct_img/normal/GTX630779/GTX630779_41631_WB_R_w_23061202_757.webp "Anti-Triosephosphate isomerase antibody [GT181] used in Western Blot (WB). GTX630779")
![Anti-Triosephosphate isomerase antibody [GT181] used in Western Blot (WB). GTX630779](/upload/website/prouct_img/normal/GTX630779/GTX630779_41631_WB_M_w_23061202_868.webp "Anti-Triosephosphate isomerase antibody [GT181] used in Western Blot (WB). GTX630779")
![Anti-Triosephosphate isomerase antibody [GT181] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630779](/upload/website/prouct_img/normal/GTX630779/GTX630779_41631_IFA_w_23061202_123.webp "Anti-Triosephosphate isomerase antibody [GT181] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630779")