GeneTex
United States (US)

Triosephosphate isomerase antibody [GT181]

KO/KD
GTX630779_41631_20161020_WB_shRNA_watermark_18121411_437.jpg
GTX630779_41631_IFA_18121411_922.jpg
GTX630779_41631_WB_R_18121411_218.jpg
GTX630779_41631_WB_M_18121411_793.jpg
GTX630779_41631_20180511_IHC-P_M_18121411_271.jpg
GTX630779_41631_WB_18121411_802.jpg

Cat No. GTX630779

Host Mouse
Clonality Monoclonal
Clone Name GT181
Isotype IgG3
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Predict Reactivity

Bovine, Dog, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Triosephosphate isomerase. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

TPI Antibody , TIM Antibody , triosephosphate isomerase 1 Antibody , TPI1 Antibody

Background

This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX630779_41631_20161020_WB_shRNA_watermark_18121411_437.jpg

GTX630779 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody [GT181] (GTX630779) diluted at 1:500.

GTX630779_41631_IFA_18121411_922.jpg

GTX630779 ICC/IF Image

Triosephosphate isomerase antibody [GT181] detects Triosephosphate isomerase protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Triosephosphate isomerase protein stained by Triosephosphate isomerase antibody [GT181] (GTX630779) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX630779_41631_WB_R_18121411_218.jpg

GTX630779 WB Image

Triosephosphate isomerase antibody [GT181] detects Triosephosphate isomerase protein by western blot analysis.
A. 30 μg PC-12 whole cell extract
B. 30 μg Rat2 whole cell extract
12 % SDS-PAGE
Triosephosphate isomerase antibody [GT181] (GTX630779) dilution: 1:1000

GTX630779_41631_WB_M_18121411_793.jpg

GTX630779 WB Image

Triosephosphate isomerase antibody [GT181] detects Triosephosphate isomerase protein by western blot analysis.
A. 30 μg Neuro2A whole cell extract
B. 30 μg C8D30 whole cell extract
C. 30 μg NIH-3T3 whole cell extract
D. 30 μg Raw264.7 whole cell extract
E. 30 μg C2C12 whole cell extract
12 % SDS-PAGE
Triosephosphate isomerase antibody [GT181] (GTX630779) dilution: 1:1000

GTX630779_41631_20180511_IHC-P_M_18121411_271.jpg

GTX630779 IHC-P Image

Triosephosphate isomerase antibody [GT181] detects Triosephosphate isomerase protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Triosephosphate isomerase stained by Triosephosphate isomerase antibody [GT181] (GTX630779) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX630779_41631_WB_18121411_802.jpg

GTX630779 WB Image

Triosephosphate isomerase antibody [GT181] detects Triosephosphate isomerase protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg A431 whole cell extract
C. 30 μg HeLa whole cell extract
D. 30 μg HepG2 whole cell extract
12 % SDS-PAGE
Triosephosphate isomerase antibody [GT181] (GTX630779) dilution: 1:1000

SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169