Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Tristetraprolin antibody (GTX130974) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tristetraprolin antibody (GTX130974) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Tristetraprolin antibody detects Tristetraprolin protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human THP-1.
Green: Tristetraprolin stained by Tristetraprolin antibody (GTX130974) diluted at 1:250.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Tristetraprolin antibody (GTX130974) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tristetraprolin antibody (GTX130974) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.