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United States (US)

Triton X-100 (25%)

Cat No. GTX30960

Application WB, ICC/IF, ELISA, IHC
Package
50 ml ($89)
See all Detergent products
PRODUCT

Product Description

Triton X-100 (25%)
APPLICATION

Application Note

For diluting antibodies use 1:1000 in buffers (1 ml into 999 ml).
Washing buffer use 1:500 (1 ml into 499 ml).
The optimum dilution should be determined by the individual lab.
PROPERTIES

Form

Liquid

Buffer

This detergent is supplied as 25% in water with 0.05 % Proclin 300 as preservative.

Storage

Room temperature.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

Non ionic detergents like Triton X-100 are used in immunoassays for diluting antibodies to lower background, and non-specific antigen-antibody (ag-ab) reaction. This detergent is also used in washing buffer from 0.1- 0.5% in all immunoassays (ELISA, Immunohistochemistry (IHC), Blot assays and others) to reduce background.Triton X-100 (polyethyleneglycolp (1,1,3,3 tetramethylbutyl) phenylether ,octylphenolethoxylate, polyoxyethylene octyl phenyl ether,4-octylphenol polyethoxylate,Mono 30, TX-100, t-octylphenoxypolyethoxyethanol, Octoxynol-9it lower surface tension of water. Triton X-100 is also used for recovery of membrane components under mild non-denaturing conditions.
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Package List Price ($)
$ 89