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TrkB antibody [2E1]

Anti-TrkB antibody [2E1] used in IHC (Paraffin sections) (IHC-P). GTX83980
Anti-TrkB antibody [2E1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83980
Anti-TrkB antibody [2E1] used in Western Blot (WB). GTX83980

Cat. No. GTX83980

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2E1

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($519)
See all TrkB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:150
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.17 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human NTRK2 expression vector

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neurotrophic receptor tyrosine kinase 2 , EIEE58 , GP145-TrkB , OBHD , TRKB , trk-B

Cellular Localization

Cell membrane,Endosome membrane,Cell projection, dendrite

Background

Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.

Database

Research Area

DATA IMAGES

Anti-TrkB antibody [2E1] used in IHC (Paraffin sections) (IHC-P). GTX83980

GTX83980 IHC-P Image

IHC-P analysis of human lung tissue using GTX83980 TrkB antibody [2E1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-TrkB antibody [2E1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83980

GTX83980 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with TrkB plasmid using GTX83980 TrkB antibody [2E1].

Anti-TrkB antibody [2E1] used in Western Blot (WB). GTX83980

GTX83980 WB Image

WB analysis of HEK293T cells transfected with TrkB plasmid (Right) or empty vector (Left) for 48 hrs using GTX83980 TrkB antibody [2E1].
Loading : 5 ug per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519