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TrkB antibody

Anti-TrkB antibody used in Western Blot (WB). GTX04764
Anti-TrkB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04764

Cat. No. GTX04764

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Mouse, Rat
Package
100 μl ($399)
See all TrkB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:50-1:200
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% glycerol

Preservative

0.05% proclin300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 32-430 of human TrkB (NP_001018074.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GP145TrkB,NTRK2,TRKB,neurotrophic receptor tyrosine kinase 2,trkB,TrkB,Trk B

Cellular Localization

Cell membrane,Endosome membrane,Early endosome membrane

Background

This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Database

Research Area

DATA IMAGES

Anti-TrkB antibody used in Western Blot (WB). GTX04764

GTX04764 WB Image

WB analysis of various samples using GTX04764 TrkB antibody.
Loading : 25 μg
Dilution : 1:1000

Anti-TrkB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04764

GTX04764 ICC/IF Image

ICC/IF analysis of Neuro-2a cells using GTX04764 TrkB antibody.
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 399